IMMOBILIZATION OF ESCHERICHIA-COLI-CELLS HAVING ASPARTASE ACTIVITY WITH CARRAGEENAN AND LOCUST BEAN GUM

Immobilization of Escherichia coli cells having aspartase activity was carried out by k{cyrillic}-carrageenan, or by k{cyrillic}-carrageenan and locust bean gum. To enhance operational stability, immobilized cells were treated with a hardening agent, such as glutaraldehyde or glutaraldehyde and hexamethylenediamine. Very active and stable immobilized preparations were obtained when E. coli cells immobilized with k{cyrillic}-carrageenan were treated with 85 mm-glutaraldehyde and 85 mm-hexamethylenediamine. The productivities of E. coli cells immobilized with polyacrylamide, k{cyrillic}-carrageenan, and k{cyrillic}-carrageenan and locust bean gum were compared for production of l-aspartic acid. Among these preparations, E. coli cells immobilized with k{cyrillic}-carrageenan and treated with glutaraldehyde and hexamethylenediamine showed the highest productivity. © 1979.