TRITIUM (H-3) RADIOLABELING OF PROTEIN A AND ANTIBODY TO HIGH SPECIFIC ACTIVITY - APPLICATION TO CELL-SURFACE ANTIGEN RADIOIMMUNOASSAYS

被引：51

作者：

WILDER, RL

YUEN, CC

SUBBARAO, B

WOODS, VL

ALEXANDER, CB

MAGE, RG

机构：

[1] Laboratory of Immunology, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 28卷 / 3-4期

关键词：

D O I：

10.1016/0022-1759(79)90192-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (>106 cpm/μg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays. © 1979.

引用

页码：255 / 266

页数：12

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