HUMAN RESPIRATORY MUCOSA IN A NONADHESIVE STATIONARY ORGAN-CULTURE SYSTEM

被引：35

作者：

STEINSVAG, SK ^{[1
]}

STRAND, M ^{[1
]}

BERG, O ^{[1
]}

MIAGUCHI, M ^{[1
]}

OLOFSSON, J ^{[1
]}

机构：

[1] KAGAWA MED SCH,DEPT OTOLARYNGOL,KAGAWA,JAPAN

来源：

LARYNGOSCOPE | 1991年 / 101卷 / 12期

关键词：

D O I：

10.1002/lary.5541011212

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Fragments of human adenoid tissue were transferred to a nonadhesive, stationary organ culture system. The culture period was 40 days. In culture, beating cilia could be observed at the surface of the fragments. Light microscopy, scanning electron microscopy, and transmission electron microscopy showed that the tissue fragments were covered by a multilayered, pseudostratified, ciliated epithelium. Beneath the epithelium was a basement membrane. At the start of the culture period, the central parts of the fragments were dominated by lymphocytes. These lymphocytes gradually disappeared and were replaced by a collagen-containing stroma with scattered fibroblasts. The tissue fragments can be used as an organ culture model for normal respiratory mucosa.

引用

页码：1323 / 1331

页数：9

共 68 条

[1] EPSTEIN-BARR VIRUS-INFECTION IN CULTURED NON-MALIGNANT EPITHELIAL-CELLS FROM HUMAN NASOPHARYNGEAL MUCOSA [J].