IDENTITY OF BCA200 AND C-ERBB-2 INDICATED BY REACTIVITY OF MONOCLONAL-ANTIBODIES WITH RECOMBINANT C-ERBB-2

被引：32

作者：

RING, DB ^{[1
]}

CLARK, R ^{[1
]}

SAXENA, A ^{[1
]}

机构：

[1] CETUS CORP,DEPT MOLEC BIOL,EMERYVILLE,CA 94608

来源：

MOLECULAR IMMUNOLOGY | 1991年 / 28卷 / 08期

关键词：

D O I：

10.1016/0161-5890(91)90056-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BCA200 has been described as a 200,000 Mr monomeric cell surface glycoprotein associated with human breast cancer. Since the physical properties and cellular distribution of BCA200 resemble those of c-erbB-2, antibodies to BCA200 were tested for the ability to bind a recombinant protein containing the c-erbB-2 extracellular domain (erbB-2 ECD). Three antibodies to distinct epitopes of BCA200 reacted with erbB-2 ECD but not with a control protein expressed in a similar baculovirus lysate. Control myeloma proteins and antibodies to four other antigens did not react with erbB-2 ECD. A protein with the expected molecular weight for erbB-2 ECD was also immunoprecipitated by anti-BCA200 antibody 520C9. We conclude that BCA200 is another synonym for c-erbB-2.

引用

页码：915 / 917

页数：3