The nucleotide sequence specifying the crp divergent RNA of Escherichia coli was determined. An open reading frame (ORF) is located at +135 to +536 relative to the initiation site of the divergent RNA. Potential factor independent transcription terminators were found at +257 to +294 and +544 to +576. These putative termination sites may account for the two RNAs of approximately 300 and 550 nucleotides previously identified as originating from the crp divergent promoter.