A NOVEL PROTEIN PHOSPHATASE INHIBITOR, TAUTOMYCIN - EFFECT ON SMOOTH-MUSCLE

被引:71
作者
HORI, M
MAGAE, J
HAN, YG
HARTSHORNE, DJ
KARAKI, H
机构
[1] UNIV TOKYO, FAC AGR, DEPT VET PHARMACOL, BUNKYO KU, TOKYO 113, JAPAN
[2] TOKYO INST TECHNOL, DEPT BIOENGN, MEGURO KU, TOKYO 152, JAPAN
[3] UNIV ARIZONA, COLL AGR, MUSCLE BIOL GRP, TUCSON, AZ 85721 USA
关键词
TAUTOMYCIN; PROTEIN PHOSPHATASE INHIBITOR; SMOOTH MUSCLE CONTRACTION;
D O I
10.1016/0014-5793(91)80745-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The antibiotic, tautomycin, was found to be a potent inhibitor of protein phosphatases and equally effective for the type-1 and type-2A enzymes. For the catalytic subunits of the type-1 and type-2A phosphatases the IC50 value was 22 to 32 nM. For the phosphatase activity present in chicken gizzard actomyosin the IC50 value was 6 nM. Tautomycin had no effect on myosin light chain kinase activity. Tautomycin induced a Ca2+-independent contraction of intact and permeabilized smooth muscle fibers and this was accompanied by an increase in the level of myosin phosphorylation. Thus, tautomycin by virtue of its ability to inhibit phosphatase activity is a valuable addition for studying the role of protein phosphorylation.
引用
收藏
页码:145 / 148
页数:4
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