微生态肠内营养对重症急性胰腺炎大鼠炎症反应和多器官损伤防护作用的研究

被引：0

作者：

王晓亮

机构：

[1] 安徽医科大学

关键词：

重症急性胰腺炎; 微生态肠内营养; MIF; ET-1; TLR-4;

D O I：

暂无

年度学位：

2010

学位类型：

硕士

导师：

汤志刚; 黄强; 陈炯; 胡何节;

摘要：

目的: 本实验通过对大鼠重症急性胰腺炎模型的建立,使用微生态肠内营养制剂对其进行干预,比较假手术组、SAP组和干预组三组各个评价指标的差异。从而评价微生态肠内营养制剂对大鼠重症急性胰腺炎炎症反应和多器官损伤防护作用的的影响。方法: 血清淀粉酶检测:经全自动生化分析仪检测血淀粉酶; 血清MIF检测:采用双抗夹心酶联免疫吸附(ELISA)法检测; RT-PCR实验:检测TLR-4在胰腺和小肠组织中的表达; ET-1、MIF在细胞中表达检测:采用SP两步法染色,检测胰腺组织、肾组织和小肠组织中的表达; 肺组织湿/干比:开胸后即取出肺右叶用电子天平称湿重后置于恒温烤箱至恒重,计算湿/干比; 组织病理学观察:胰腺组织、肺组织、肾脏组织和小肠组织进行HE染色,按照Gross评分原则对其评分。结果: 成功构建了重症急性胰腺炎大鼠合并多器官损伤的模型; 对于血清淀粉酶和MIF,假手术组明显低于SAP组和干预组;SAP组与干预组比较则高于干预组; 假手术组胰腺组织和小肠组织中ET-1、MIF在细胞中未见明显表达,SAP组中阳性率高,干预组胰腺中ET-1、MIF阳性率表达明显低于SAP组; RT-PCR结果显示,TLR4在假手术组中表达量很少,在SAP组中表达量较大,干预组表达较SAP组量少; 假手术组肺组织湿/干比明显低于干预组和SAP组,SAP组肺组织湿/干比则高于干预组; 假手术组胰腺组织坏死不明显,SAP组胰腺坏死程度高于干预组。假手术组各器官损害不明显,SAP组个器官组织损害明显,SAP组病理学评分明显高于干预组。结论: 微生态肠内营养制剂能显著降低重症急性胰腺炎大鼠炎症反应,降低血清淀粉酶和MIF,降低ET-1和MIF的表达,降低肺组织的湿干比值,改善多器官功能的损害,减轻胰腺组织炎症损伤。

引用

页数：51

共 36 条

[1]

Ulinastatin suppresses systemic inflammatory response following lung ischemia-reperfusion injury in rats [J].

Xu, L. ;

Ren, B. ;

Li, M. ;

Jiang, F. ;

Zhanng, Z. ;

Hu, J. .

TRANSPLANTATION PROCEEDINGS, 2008, 40 (05) :1310-1311

[2]

Effect of enteral nutrition and synbiotics on bacterial infection rates after pylorus-preserving pancreatoduodenectomy -: A randomized, double-blind trial [J].

Rayes, Nada ;

Seehofer, Daniel ;

Theruvath, Tom ;

Mogl, Martina ;

Langrehr, Jan M. ;

Nuessler, Natascha C. ;

Bengmark, Stig ;

Neuhaus, Peter .

ANNALS OF SURGERY, 2007, 246 (01) :36-41

[3]

Modification of intestinal flora with multispecies probiotics reduces bacterial translocation and improves clinical course in a rat model of acute pancreatitis [J].

van Minnen, L. Paul ;

Timmerman, Harro M. ;

Lutgendorff, Femke ;

Verheem, Andre ;

Harmsen, Wil ;

Konstantinov, Sergey R. ;

Smidt, Hauke ;

Visser, Maarten R. ;

Rijkers, Ger T. ;

Gooszen, Hein G. ;

Akkermans, Louis M. A. .

SURGERY, 2007, 141 (04) :470-480

[4]

Immunomodulation in surgical practice.[J].R. Andersson;B. Andersson;E. Andersson;G. Eckerwall;M. Nordén;B. Tingstedt.HPB.2006, 2

[5]

Significant elevation of serum interleukin-18 levels in patients with acute pancreatitis.[J].Takashi Ueda;Yoshifumi Takeyama;Takeo Yasuda;Naoki Matsumura;Hidehiro Sawa;Takahiro Nakajima;Tetsuo Ajiki;Yasuhiro Fujino;Yasuyuki Suzuki;Yoshikazu Kuroda.Journal of Gastroenterology.2006, 2

[6]

Randomized clinical trial of specific lactobacillus and fibre supplement to early enteral nutrition in patients with acute pancreatitis [J].

Oláh, A ;

Belágyi, T ;

Issekutz, A ;

Gamal, ME ;

Bengmark, S .

BRITISH JOURNAL OF SURGERY, 2002, 89 (09) :1103-1107

[7]

Altered intestinal morphology and immunity in patients with acute necrotizing pancreatitis.[J].Basil J. Ammori;Alison Cairns;Michael F. Dixon;Michael Larvin;Michael J. McMahon.Journal of Hepato-Biliary-Pancreatic Surgery.2002, 4

[8]

Nutrition support during acute pancreatitis.[J].Souheil Abou-Assi;Stephen J.D O’Keefe.Nutrition.2002, 11

[9]

Early nasojejunal feeding in acute pancreatitis is associated with a lower complication rate [J].