凉水、丰林国家自然保护区天然红松林遗传变异的研究

被引:0
作者
吴隆坤
机构
[1] 辽宁师范大学
关键词
红松; RAPD; ISSR; 遗传多样性;
D O I
暂无
年度学位
2005
学位类型
硕士
导师
摘要
本文应用RAPD 和ISSR 技术对凉水和丰林国家自然保护区的天然红松(Pinus koraiensis )居群的共74 个个体遗传多样性变化和遗传分化进行了分析。10 个RAPD 随机引物共检测到61 个位点, 其中多态位点43个, 多态位点比率为69.35%。RAPD 的Shannon指数凉水居群为39.70%,丰林居群为37.35%, Nei 指数统计结果表明, 红松的遗传多样性凉水居群(0.2634)大于丰林居群(0.2607)。13 个ISSR 随机引物共检测到103 个可重复的位点,其中74 个位点具有多态性,多态位点百分比为分别为75.25%和74.00%。ISSR 的Shannon 指数凉水和丰林两个居群分别为43.95%和40.61%,ISSR 的Nei 指数遗传分化为5.63%。RAPD 和ISSR 的两种指数都表明, 红松的遗传多样性凉水居群大于丰林居群,红松种内的遗传变异主要存在于群体内,居群内存在一定的遗传分化。研究表明红松在松科植物中的遗传多样性水平是不低的。由此说明红松物种濒危、分布区逐渐缩小的原因,归根结底是由于人类的破坏作用,再加上火灾和风倒等因素造成的。
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页数:48
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