NO处理对草莓果实贮藏性及活性氧代谢的影响

被引:0
作者
李善菊
机构
[1] 西北农林科技大学
关键词
草莓; NO; 衰老; 活性氧代谢; 贮藏;
D O I
暂无
年度学位
2005
学位类型
硕士
导师
摘要
本文以“红太后”草莓果实为试材,研究外源一氧化氮(NO)处理对草莓果实室温下贮藏保鲜的效果,探讨其对果实成熟衰老中各因子如:乙烯释放量、呼吸速率、相对膜透性、可溶性糖含量、可滴定酸含量、可溶性固形物含量、可溶性蛋白含量、叶绿素含量、花青苷含量、Vc 含量、果实硬度、果实的失重、MDA 含量等的变化特性,以及NO 对果实活性氧代谢及其活性氧代谢相关酶类如SOD、CAT、POD、APX 等活性的影响。初步探讨了NO 处理对草莓果实成熟衰老影响的生理生化机制,为进一步开发NO 在果实保鲜上的应用提供理论依据。 实验结果表明: (1)外源NO 处理延缓了草莓果实在贮藏期间硬度的下降,抑制了果实叶绿素的降解、花青苷的合成,延缓了果实颜色的转变,降低了果实水分的散失,减少了果实的腐烂率,延缓了蛋白质和Vc 的降解,抑制了贮藏前期可滴定酸的下降和可溶性糖的变化,降低了膜透性,减缓了可溶性固形物的变化速率,从而在一定程度上保持了果实的新鲜度,保持了草莓果实的品质,延缓了草莓果实的衰老。表明外源NO 对草莓具有良好的保鲜作用,进一步证明NO 可以成为一种理想的保鲜剂。 (2)外源NO 处理可以抑制加速果实衰老的H2O2、O2-.含量的积累,提高SOD、POD、CAT、APX 等保护酶系的活性。 (3)外源NO 处理可降低草莓果实贮藏期间的呼吸速率和减少乙烯释放量。 (4)外源NO 处理可以抑制外源乙烯诱导的果实硬度下降、呼吸速率及乙烯生成量的增加、花青苷的合成、叶绿素的降解;NO 在一定程度上抑制乙烯诱导的H2O2、O2-.含量的上升,并对乙烯诱导的清除活性氧的保护酶类(SOD、POD、CAT、APX)的活性降低有一定的抑制作用。
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