PHOSPHOLIPID PROFILES OF HUMAN COLON CANCER USING P-31 MAGNETIC-RESONANCE SPECTROSCOPY

被引：70

作者：

MERCHANT, TE

KASIMOS, JN

DEGRAAF, PW

MINSKY, BD

GIERKE, LW

GLONEK, T

机构：

[1] Department of Pathology, University Hospital Utrecht, Utrecht

[2] MR Laboratory, Chicago College of Osteopathic Medicine, Chicago, Illinois

[3] Department of Pathology, Chicago College of Osteopathic Medicine, Chicago, Illinois

[4] Department of Surgery, University Hospital Utrecht, Utrecht

[5] Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York

来源：

INTERNATIONAL JOURNAL OF COLORECTAL DISEASE | 1991年 / 6卷 / 02期

关键词：

D O I：

10.1007/BF00300208

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Phospholipids of 16 malignant and 11 non-malignant human colon specimens were analyzed using a chloroform-methanol analytical reagent in conjunction with P-31 magnetic resonance spectroscopy (MRS) at 202.4 MHz. Sixteen individual generic phospholipids were identified and quantified for statistical intergroup comparisons. Statistically significant elevations in the relative concentrations of lysophosphatidylcholine and phosphatidylcholine plasmalogen were seen in malignant tissues along with significantly depressed levels of sphingomyelin and phosphatidylethanolamine plasmalogen. The malignant and non-malignant tissue groups were further differentiated by the detection of the minor phospholipids, lysophosphatidylcholine plasmalogen, lysophosphatidylethanolamine plasmalogen, lysophosphatidic acid and phosphatidylglycerol exclusively present in the malignant tissues and by significant changes in computed phospholipid metabolic indices that were dominated by choline containing lipids. The P-31 MRS methods used represent an advancement over previous protocols for identifying and quantifying major and minor tissue phospholipids making this the first direct study of membrane phospholipids in human colon tissues using P-31 MRS. The phospholipid profiles obtained may provide important information regarding the nature of the malignant cell's membrane system and identify markers which may be used to estimate malignant propensity, aggressiveness of disease and provide prognostic information.

引用

页码：121 / 126

页数：6

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