CONSTITUTIVE AND INDUCIBLE EXPRESSION OF A TRANSGENE DIRECTED BY HETEROLOGOUS PROMOTERS IN A TROUT LIVER-CELL LINE

Activities of heterologous promoters and enhancers in cultured rainbow trout liver cells were examined employing the bacterial chloramphenicol acetyltransferase gene as the reporter. SV40 promoter-enhancer and Rous sarcoma virus long terminal repeat directed constitutive expression at high levels. Moloney murine leukemia virus long terminal repeat and SV40 promoter combined with Adenovirus type 2 enhancer were also constitutively expressed. Drosophila Hsp70 promoter was activated when the transformed cells were cultured at 25 °C, a higher temperature than the temperature normally used, in faithful response to heat shock. © 1990 Academic Press, Inc.