NEW SITE-SPECIFIC ENDONUCLEASE FROM STREPTOMYCES-LAVENDULAE (SLAI)

被引：27

作者：

TAKAHASHI, H ^{[1
]}

SHIMIZU, M ^{[1
]}

SAITO, H ^{[1
]}

IKEDA, Y ^{[1
]}

SUGISAKI, H ^{[1
]}

机构：

[1] KYOTO UNIV,INST CHEM RES,UJI,KYOTO 611,JAPAN

来源：

GENE | 1979年 / 5卷 / 01期

关键词：

Bacteriophage; λ; CTCGAG; recombinant DNA cytosine-substituted T4 DNA; XhoI isoschizomer;

D O I：

10.1016/0378-1119(79)90089-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A new restriction-like endonuclease, SlaI, was found and partially purified from Streptomyces lavendulae ATCC8664. This endonuclease cleaved bacteriophage lambda DNA at only one site, and cytosine-substituted bacteriophage T4 DNA at 16 sites. The recognition sequence was determined by using SlaI fragments of cytosine-substituted bacteriophage T4 DNA. The hexanucleotide recognized by SlaI endonuclease was 5′-C{downwards double arrow}T-C-G-A-G-3′3′-G-A-G-C-A-↑C-5′ with the sites of cleavage as indicated by the arrows. Therefore, SlaI endonuclease was an isochizomer of XhoI endonuclease. © 1979.

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页码：9 / 18

页数：10

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