KINETIC-PROPERTIES OF SHEEP BRAIN GLUTATHIONE-REDUCTASE

被引:12
作者
ACAN, NL
TEZCAN, EF
机构
关键词
GLUTATHIONE REDUCTASE; ENZYME KINETICS; SHEEP BRAIN;
D O I
10.1159/000468877
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The kinetic properties of sheep brain glutathione reductase (GSSGR) were investigated. The enzyme showed Ping-Pong kinetics with double substrate inhibition in the forward direction. K(m) values for NADPH and GSSG were found to be 60.9 and 116.9-mu-mol/l, and K(i) values were found to be 42.1 and 347.3-mu-mol/l, respectively. NADP+ inhibition at low fixed concentration of NADPH was mixed-type with a K(i) of 281.5-mu-mol/l and alpha of 0.048. It is concluded that the enzyme shows a hybrid Ping-Pong-ordered branched mechanism.
引用
收藏
页码:121 / 124
页数:4
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