BINDING-KINETICS OF ANTIBODIES TO ANTIGENS ON POLYMER SURFACES AS STUDIED BY THE MULTIPLE INTERNAL-REFLECTION FLUORESCENCE METHOD

被引:14
作者
TANIMOTO, S
KITANO, H
机构
[1] TOYAMA UNIV,DEPT CHEM & BIOCHEM ENGN,TOYAMA 930,JAPAN
[2] KYOTO UNIV,DEPT POLYMER CHEM,KYOTO 606,JAPAN
关键词
D O I
10.1021/la00029a026
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Binding processes of an antibody (anti-human serum albumin-immunoglobulin G) labeled with fluorescein (FITC-anti-HSA-IgG) to an antigen (HSA) bound to polymer surfaces were followed by the multiple internal reflection fluorescence (MIRF) method. The antigen (HSA) was adsorbed strongly onto hydrodrophobic polymer surfaces (poly(methyl methacrylate) (PMMA)), and the protein adsorbed was scarcely removed by rinsing with a buffer solution. The initial rate of the binding of FITC-anti-HSA-IgG to the antigen on the polymer surface was very large (about 10% of the diffusion-controlled association at the most), whereas there was no binding of the antibody to the surface modified with ovalbumin (OVA). The effects of surface density of the antigen on the binding rate and the amount of antibody bound were examined.
引用
收藏
页码:1315 / 1318
页数:4
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