RESONANCE RAMAN-SPECTROSCOPIC STUDIES OF ENZYME-SUBSTRATE INTERMEDIATES AT 5-K

被引：9

作者：

KIM, MS ^{[1
]}

CAREY, PR ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,INST BIOL SCI,OTTAWA K1A 0R6,ONTARIO,CANADA

来源：

JOURNAL OF MOLECULAR STRUCTURE | 1991年 / 242卷

关键词：

D O I：

10.1016/0022-2860(91)87152-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A simple and versatile system for resonance Raman (RR) spectroscopic analysis of enzyme-substrate complexes at liquid helium temperatures is described. The system allows us to record high-quality RR spectra for dithioacyl papain intermediates (MeO-Phe-Gly- and MeO-Gly-Gly-Phe-Gly-C(= S)S-papain) in ice matrices at 5 K. Based on established structure-spectra correlations, it is concluded that the active-site conformation of the intermediates about the phi', psi' glycinic linkages and cysteine-25 side chain is B-G+-P(H) both in ice matrices at 5 K and in solution at room temperature.

引用

页码：421 / 431

页数：11

共 18 条

[11] C-S STRETCHING VIBRATIONS AND MOLECULAR-CONFORMATIONS OF METHYL PROPYL SULFIDE AND RELATED ALKYL SULFIDES [J].

NOGAMI, N ;

SUGETA, H ;

MIYAZAWA, T .

CHEMISTRY LETTERS, 1975, (02) :147-150

[12] PHOTODISSOCIATED CYTOCHROME-C OXIDASE - CRYOTRAPPED METASTABLE INTERMEDIATES [J].

SASSAROLI, M ;

CHING, YC ;

ARGADE, PV ;

ROUSSEAU, DL .

BIOCHEMISTRY, 1988, 27 (07) :2496-2502

[13] BACKSCATTERING GEOMETRY FOR RAMAN-SPECTROSCOPY OF COLORED MATERIALS [J].

SHRIVER, DF ;

DUNN, JBR .

APPLIED SPECTROSCOPY, 1974, 28 (04) :319-323

[14] RELAXED AND PERTURBED SUBSTRATE CONFORMATIONS IN ENZYME ACTIVE-SITES - EVIDENCE FROM MULTICHANNEL RESONANCE RAMAN-SPECTRA [J].

STORER, AC ;

LEE, H ;

CAREY, PR .

BIOCHEMISTRY, 1983, 22 (20) :4789-4796

[15]

STORER AC, 1979, J BIOL CHEM, V254, P3163

[16] STRIKING CHANGES OBSERVED IN KEY ACYL-ENZYME LINKAGES BY RESONANCE RAMAN EXPERIMENTS NEAR 77-K [J].

TONGE, PJ ;

LEE, H ;

CARTIER, LRS ;

RUZSICSKA, BP ;

CAREY, PR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (04) :1496-1497

[17] DIRECTIONAL PREFERENCE FOR A CATALYTICALLY IMPORTANT N...S CONTACT SEEN IN ACYL-THIOLPROTEASES [J].

VARUGHESE, KI ;

STORER, AC ;

CAREY, PR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (26) :8252-8257

[18] SPINNING CELL FOR LOW-TEMPERATURE RESONANCE RAMAN-SPECTRA WITHOUT DEWAR LOSSES [J].

WALTERS, MA .

APPLIED SPECTROSCOPY, 1983, 37 (03) :299-300

← 1 2 →