INHIBITION OF HIV-1 IN-VITRO BY C-5 PROPYNE PHOSPHOROTHIOATE ANTISENSE TO REV

被引：7

作者：

LUND, OS ^{[1
]}

NIELSEN, JO ^{[1
]}

HANSEN, JES ^{[1
]}

机构：

[1] UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT 144,INFECT DIS LAB,DK-2650 HVIDOVRE,DENMARK

来源：

ANTIVIRAL RESEARCH | 1995年 / 28卷 / 01期

关键词：

HUMAN IMMUNODEFICIENCY VIRUS (HIV); PHOSPHOROTHIOATE; C-5; PROPYNE; ANTISENSE; REV; CHRONIC INFECTION;

D O I：

10.1016/0166-3542(95)00041-J

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A 15-mer C-5 propyne modified phosphorothioate oligodeoxynucleotide antisense to rev was approximately 5-fold more effective in providing viral inhibition compared to a 28-mer unmodified phosphorothioate oligodeoxynucleotide targeted to the same sequence and previously shown to inhibit HIV-1 in a sequence-dependent manner. The antiviral effect was obtained by lipofection or simple addition of 0.2-1 mu M modified oligodeoxynucleotide to the culture medium of H9 cells chronically infected with the HIV-1(LAI) isolate of human immunodeficiency virus type 1. We conclude that C-5 propyne oligodeoxynucleotides in accordance with previous findings by others are superior to unmodified phosphorothioates in providing inhibition of HIV-1 in a sequence-dependent manner and that this inhibition can be conferred by oligodeoxynucleotides in free solution.

引用

页码：81 / 91

页数：11