BIOCONVERSION OF PIMELIC ACID INTO BIOTIN BY BACILLUS-SPHAERICUS BIOB TRANSFORMANTS

被引：8

作者：

OHSAWA, I ^{[1
]}

KISOU, T ^{[1
]}

KODAMA, K ^{[1
]}

YONEDA, I ^{[1
]}

SPECK, D ^{[1
]}

GLOECKLER, R ^{[1
]}

LEMOINE, Y ^{[1
]}

KAMOGAWA, K ^{[1
]}

机构：

[1] TRANSGENE SA,F-67082 STRASBOURG,FRANCE

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 73卷 / 02期

关键词：

D O I：

10.1016/0922-338X(92)90475-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus sphaericus was treated with N-acetylmuramidase and transformed with different bioB expression vectors by protoplast transformation, of which only three could be introduced into B. sphaericus. After cultivating the transformants for 24 h at 28-degrees-C, the culture was supplemented with pimelic acid and incubated for 48 h at 37-degrees-C. In these conditions, B. sphaericus IFO3525 transformed with a vector containing bioB under the control of the "HpaII promoter" on pUB110 accumulated 20-mu-g of biotin/ml in the medium.

引用

页码：121 / 124

页数：4

共 24 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] HIGH-FREQUENCY TRANSFORMATION OF BACILLUS-SUBTILIS PROTOPLASTS BY PLASMID DNA [J].