DEVELOPMENT OF AN ISOTOPE-SELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DETECTOR USING CHEMICAL-REACTION-INTERFACE MASS-SPECTROMETRY - APPLICATION TO DEUTERATED CORTISOL METABOLITES IN URINE

被引：25

作者：

TEFFERA, Y

ABRAMSON, FP

MCLEAN, M

VESTAL, M

机构：

[1] GEORGE WASHINGTON UNIV,MED CTR,DEPT PHARMACOL,WASHINGTON,DC 20037

[2] VESTEC,HOUSTON,TX 77054

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 620卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80055-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An isotope-selective detector for HPLC based on the particle-beam-interface and the chemical-reaction-interface mass spectrometer (CRIMS) principle is described. This paper focuses on the selective detection of deuterium-labeled analytes. The CRIMS product HD is detected as has been previously described for GC-CRIMS. The analytical performance was not affected by analyte structure or solvent composition. Deuterium detection was linear from 20 ng to 490 ng using H-2(3)-labeled cortisol. Based on this method, the fractional abundance of three labeled metabolites of cortisol were determined in the urine of a patient infused with tracer amounts of deuterated cortisol.

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收藏

页码：89 / 96

页数：8

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