OPTIMIZATION OF SKIMMER VOLTAGES OF AN ELECTROSPRAY ION-SOURCE COUPLED WITH A MAGNETIC-SECTOR INSTRUMENT

被引:13
作者
MURATA, H [1 ]
TAKAO, T [1 ]
SHIMONISHI, Y [1 ]
机构
[1] OSAKA UNIV,INST PROTEIN RES,YAMADAOKA 3-2,SUITA,OSAKA 565,JAPAN
关键词
D O I
10.1002/rcm.1290080216
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An electrospray ion source designed for a magnetic sector instrument usually has two skimmers, which are located behind a capillary exit with separate pumping stages to reduce the pressure from that of ambient air to a valve suitable for the sector instrument. This work describes an evaluation of these two skimmer voltages with respect to the charge-state distributions and the focusing efficiency of ions. The skimmer voltages were found to be closely related to the m/z values of the detected ions. The optimal difference voltage between skimmers 1 and 2 increased in proportion to the m/z values of the ions, irrespective of the molecular masses of the associated substances. This phenomenon is ascribable to the ion-focusing effect in this region rather than to the effects of energetic collisions in the region of the skimmers, and suggests that the skimmer voltages should be adjusted in accordance with the m/z values of the detected ions for efficient acquisition of all distributed ions. The work demonstrates the functional role of the skimmers in electrospray ionization mass spectrometry with a magnetic sector instrument by examining several types of peptides and proteins, as well as an analysis of a complex mixture of peptides.
引用
收藏
页码:205 / 210
页数:6
相关论文
共 13 条
[1]   ELECTROSPRAY IONIZATION MAGNETIC-SECTOR MASS-SPECTROMETRY - CALIBRATION, RESOLUTION, AND ACCURATE MASS MEASUREMENTS [J].
CODY, RB ;
TAMURA, J ;
MUSSELMAN, BD .
ANALYTICAL CHEMISTRY, 1992, 64 (14) :1561-1570
[2]   STRUCTURAL CHARACTERIZATION OF PROTEIN TRYPTIC PEPTIDES VIA LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND COLLISION-INDUCED DISSOCIATION OF THEIR DOUBLY CHARGED MOLECULAR-IONS [J].
COVEY, TR ;
HUANG, EC ;
HENION, JD .
ANALYTICAL CHEMISTRY, 1991, 63 (13) :1193-1200
[3]   CAPILLARY ELECTROPHORESIS ELECTROSPRAY IONIZATION-MASS SPECTROMETRY [J].
EDMONDS, CG ;
LOO, JA ;
BARINAGA, CJ ;
UDSETH, HR ;
SMITH, RD .
JOURNAL OF CHROMATOGRAPHY, 1989, 474 (01) :21-37
[4]   ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
SCIENCE, 1989, 246 (4926) :64-71
[5]   DESIGN AND PERFORMANCE OF AN ELECTROSPRAY IONIZATION SOURCE FOR A DOUBLY-FOCUSING MAGNETIC-SECTOR MASS-SPECTROMETER [J].
GALLAGHER, RT ;
CHAPMAN, JR ;
MANN, M .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1990, 4 (10) :369-372
[6]   ACCURATE MASS MEASUREMENT OF PROTEINS USING ELECTROSPRAY IONIZATION ON A MAGNETIC-SECTOR INSTRUMENT [J].
MCEWEN, CN ;
LARSEN, BS .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (03) :173-178
[7]   ION SPRAY LIQUID-CHROMATOGRAPHY ION TRAP MASS-SPECTROMETRY DETERMINATION OF BIOMOLECULES [J].
MCLUCKEY, SA ;
VANBERKEL, GJ ;
GLISH, GL ;
HUANG, EC ;
HENION, JD .
ANALYTICAL CHEMISTRY, 1991, 63 (04) :375-383
[8]   ELECTROSPRAY IONIZATION ON A HIGH-PERFORMANCE MAGNETIC-SECTOR MASS-SPECTROMETER [J].
MENG, CK ;
MCEWEN, CN ;
LARSEN, BS .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1990, 4 (05) :147-150
[9]   ONLINE MEMBRANE BLOTTING OF PEPTIDES AND PROTEINS FROM A CAPILLARY COLUMN [J].
MURATA, H ;
TAKAO, T ;
ANAHARA, S ;
SHIMONISHI, Y .
ANALYTICAL BIOCHEMISTRY, 1993, 210 (01) :206-208
[10]   ONLINE MASS-SPECTROMETRIC DETECTION FOR CAPILLARY ZONE ELECTROPHORESIS [J].
OLIVARES, JA ;
NGUYEN, NT ;
YONKER, CR ;
SMITH, RD .
ANALYTICAL CHEMISTRY, 1987, 59 (08) :1230-1232