CHARACTERIZATION OF AARA, A PLEIOTROPIC NEGATIVE REGULATOR OF THE 2'-N-ACETYLTRANSFERASE IN PROVIDENCIA-STUARTII

被引:36
作者
RATHER, PN
OROSZ, E
机构
[1] CASE WESTERN RESERVE UNIV,SCH MED,DEPT MED,CLEVELAND,OH 44106
[2] CASE WESTERN RESERVE UNIV,SCH MED,DEPT MOLEC BIOL & MICROBIOL,CLEVELAND,OH 44106
[3] VET AFFAIRS MED CTR,RES SERV,CLEVELAND,OH 44106
关键词
D O I
10.1128/jb.176.16.5140-5144.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have utilized transposon mutagenesis to obtain insertional mutations in Providencia stuartii that activate the chromosomal aac(2')-la gene. Two closely linked mini-Tn5Cm insertions were obtained in a locus designated aarA, and a single insertion was obtained in a separate locus, aarC. Nucleotide sequence analysis, complementation studies, and localization of the sites of mini-Tn5Cm insertion have allowed the identification of the aarA coding region. The deduced AarA protein had a molecular mass of 31,086 kDa and displayed characteristics of an integral membrane protein. A strain deleted for the aarA gene by allelic exchange showed at least a fourfold increase in the accumulation of aac(2')-la mRNA and an eightfold increase in aminoglycoside resistance. Mutations in aarA were pleiotrophic and also resulted in loss of pigmentation and a deficiency in cell separation during division.
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页码:5140 / 5144
页数:5
相关论文
共 33 条
[1]  
Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210
[2]   PURIFICATION AND MUTANT ANALYSIS OF CITROBACTER-FREUNDII AMPR, THE REGULATOR FOR CHROMOSOMAL AMPC BETA-LACTAMASE [J].
BARTOWSKY, E ;
NORMARK, S .
MOLECULAR MICROBIOLOGY, 1991, 5 (07) :1715-1725
[3]   POSTTRANSCRIPTIONAL REPRESSION OF ESCHERICHIA-COLI OMPF PROTEIN IN RESPONSE TO REDOX STRESS - POSITIVE CONTROL OF THE MICF ANTISENSE RNA BY THE SOXRS LOCUS [J].
CHOU, JH ;
GREENBERG, JT ;
DEMPLE, B .
JOURNAL OF BACTERIOLOGY, 1993, 175 (04) :1026-1031
[4]   MARA LOCUS CAUSES DECREASED EXPRESSION OF OMPF PORIN IN MULTIPLE-ANTIBIOTIC-RESISTANT (MAR) MUTANTS OF ESCHERICHIA-COLI [J].
COHEN, SP ;
MCMURRY, LM ;
LEVY, SB .
JOURNAL OF BACTERIOLOGY, 1988, 170 (12) :5416-5422
[5]   CROSS-RESISTANCE TO FLUOROQUINOLONES IN MULTIPLE-ANTIBIOTIC-RESISTANT (MAR) ESCHERICHIA-COLI SELECTED BY TETRACYCLINE OR CHLORAMPHENICOL - DECREASED DRUG ACCUMULATION ASSOCIATED WITH MEMBRANE-CHANGES IN ADDITION TO OMPF REDUCTION [J].
COHEN, SP ;
MCMURRY, LM ;
HOOPER, DC ;
WOLFSON, JS ;
LEVY, SB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (08) :1318-1325
[6]   GENETIC AND FUNCTIONAL-ANALYSIS OF THE MULTIPLE ANTIBIOTIC-RESISTANCE (MAR) LOCUS IN ESCHERICHIA-COLI [J].
COHEN, SP ;
HACHLER, H ;
LEVY, SB .
JOURNAL OF BACTERIOLOGY, 1993, 175 (05) :1484-1492
[7]   MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS, PROMOTER PROBING, AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA [J].
DELORENZO, V ;
HERRERO, M ;
JAKUBZIK, U ;
TIMMIS, KN .
JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6568-6572
[8]   CONSTRUCTION OF BROAD-HOST-RANGE PLASMID VECTORS FOR EASY VISIBLE SELECTION AND ANALYSIS OF PROMOTERS [J].
FARINHA, MA ;
KROPINSKI, AM .
JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :3496-3499
[9]   OVEREXPRESSION OF THE MARA POSITIVE REGULATOR IS SUFFICIENT TO CONFER MULTIPLE ANTIBIOTIC-RESISTANCE IN ESCHERICHIA-COLI [J].
GAMBINO, L ;
GRACHECK, SJ ;
MILLER, PF .
JOURNAL OF BACTERIOLOGY, 1993, 175 (10) :2888-2894
[10]   POSITIVE SELECTION PROCEDURE FOR ENTRAPMENT OF INSERTION-SEQUENCE ELEMENTS IN GRAM-NEGATIVE BACTERIA [J].
GAY, P ;
LECOQ, D ;
STEINMETZ, M ;
BERKELMAN, T ;
KADO, CI .
JOURNAL OF BACTERIOLOGY, 1985, 164 (02) :918-921