WHEAT-GERM-AGGLUTININ CHROMATOGRAPHY OF GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL AND GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC, OBTAINED BY INVITRO SYNTHESIS AND BY PARTIAL CLEAVAGE OF TERATOCARCINOMA POLY-N-ACETYLLACTOSAMINOGLYCANS

被引：29

作者：

SEPPO, A ^{[1
]}

PENTTILA, L ^{[1
]}

MAKKONEN, A ^{[1
]}

LEPPANEN, A ^{[1
]}

NIEMELA, R ^{[1
]}

JANTTI, J ^{[1
]}

HELIN, J ^{[1
]}

RENKONEN, O ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT BIOCHEM,SF-00170 HELSINKI 17,FINLAND

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1990年 / 68卷 / 01期

关键词：

D O I：

10.1139/o90-006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GlcNAcβ1-3(GlcNAcβ1-6)[14C(U)]Gal and GlcNAcβ1-3(GlcNAcβ1-6) [14C(U)]Galβ1-4GlcNAc were prepared by in vitro synthesis. They were characterized by enzymatic sequencing, by partial acid hydrolysis, and by periodate oxidation experiments. The two saccharides were isolated also from partial acid hydrolysates of metabolically labeled poly-N-acetyllactosaminoglycans of murine embryonal carcinoma cells (line PC 13). The tetrasaccharide was retarded in a column of agarose-linked wheat germ agglutinin; the trisaccharide was strongly bound. Chromatography in this column separated the trisaccharide into two distinct peaks, which represented interconvertible molecules. Together with our previous data on linear teratocarcinoma saccharides, these findings show that affinity chromatography with immobilized wheat germ agglutinin can be advantageously used in fractionating radiolabeled oligo-N-acetyllactosaminoglycans and saccharides related to them.

引用

页码：44 / 53

页数：10

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