X-RAY STUDIES REVEAL LANTHANIDE BINDING-SITES AT THE A/B5 INTERFACE OF ESCHERICHIA-COLI HEAT LABILE ENTEROTOXIN

被引：8

作者：

SIXMA, TK ^{[1
]}

VANSCHELTINGA, ACT ^{[1
]}

KALK, KH ^{[1
]}

ZHOU, K ^{[1
]}

WARTNA, ES ^{[1
]}

HOL, WGJ ^{[1
]}

机构：

[1] UNIV GRONINGEN, BIOSON RES INST, DEPT CHEM, NIJENBORGH 4, 9747 AG GRONINGEN, NETHERLANDS

来源：

FEBS LETTERS | 1992年 / 297卷 / 1-2期

关键词：

HEAT LABILE ENTEROTOXIN; CHOLERA TOXIN; CALCIUM; LANTHANIDE ION; CONFORMATIONAL CHANGE;

D O I：

10.1016/0014-5793(92)80355-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure determination of heat labile enterotoxin (LT) bound to two different lanthanide ions, erbium and samarium, revealed two distinct ion binding sites in the interface of the A subunit and the B pentamer of the toxin. One of the interface sites is conserved in the very similar cholera toxin sequence. These sites may be potential calcium binding sites. Erbium and samarium binding causes a change in the structure of LT: a rotation of the Al subunit of up to two degrees relative to the B pentamer.

引用

页码：179 / 182

页数：4

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