A NEW CONUS PEPTIDE LIGAND FOR MAMMALIAN PRESYNAPTIC CA2+ CHANNELS

被引：461

作者：

HILLYARD, DR

MONJE, VD

MINTZ, IM

BEAN, BP

NADASDI, L

RAMACHANDRAN, J

MILJANICH, G

AZIMIZOONOOZ, A

MCINTOSH, JM

CRUZ, LJ

IMPERIAL, JS

OLIVERA, BM

机构：

[1] UNIV UTAH,DEPT PATHOL,SALT LAKE CITY,UT 84132

[2] UNIV UTAH,DEPT PSYCHIAT,SALT LAKE CITY,UT 84132

[3] HARVARD UNIV,SCH MED,DEPT NEUROBIOL,BOSTON,MA 02115

[4] UNIV PHILIPPINES,INST CHEM,QUEZON CITY 1107,PHILIPPINES

[5] UNIV PHILIPPINES,INST MARINE SCI,QUEZON CITY 1107,PHILIPPINES

[6] NEUREX CORP,MENLO PK,CA 94025

来源：

NEURON | 1992年 / 9卷 / 01期

关键词：

D O I：

10.1016/0896-6273(92)90221-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Voltage-sensitive Ca2+ channels that control neurotransmitter release are blocked by omega-conotoxin (omega-CgTx) GVIA from the marine snail Conus geographus, the most widely used inhibitor of neurotransmitter release. However, many mammalian synapses are omega-CgTx-GVIA insensitive. We describe a new Conus peptide, omega-CgTx-MVIIC, that is an effective inhibitor of omega-CgTx-GVIA-resistant synaptic transmission. Ca2+ channel targets that are inhibited by omega-CgTx-MVIIC but not by omega-CgTx-GVIA include those mediating depolarization-induced Ca-45(2+) uptake in rat synaptosome preparations, "P" currents in cerebellar Purkinje cells, and a subset of omega-CgTx-GVIA-resistant currents in CA1 hippocampal pyramidal cells. The characterization of omega-CgTx-MVIIC by a combination of molecular genetics and chemical synthesis defines a general approach for obtaining ligands with novel receptor subtype specificity from Conus.

引用

页码：69 / 77

页数：9

共 24 条

[1] EFFECTS OF POTASSIUM, VERATRIDINE AND SCORPION-VENOM ON CALCIUM ACCUMULATION AND TRANSMITTER RELEASE BY NERVE-TERMINALS INVITRO
BLAUSTEIN, MP
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1975, 247 (03): : 617 - 655
[2] NEUROTOXINS THAT ACT ON VOLTAGE-SENSITIVE SODIUM-CHANNELS IN EXCITABLE-MEMBRANES
CATTERALL, WA
[J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1980, 20 : 15 - 43
[3] CRUZ LJ, 1986, J BIOL CHEM, V261, P6230
[4] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES
HAMILL, OP
MARTY, A
NEHER, E
SAKMANN, B
SIGWORTH, FJ
[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02): : 85 - 100
[5] Katz B, 1969, RELEASE NEURAL TRANS
[6] BLOCKING AND ISOLATION OF A CALCIUM-CHANNEL FROM NEURONS IN MAMMALS AND CEPHALOPODS UTILIZING A TOXIN FRACTION (FTX) FROM FUNNEL-WEB SPIDER POISON
LLINAS, R
SUGIMORI, M
LIN, JW
CHERKSEY, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) : 1689 - 1693
[7] PHARMACOLOGICAL EVIDENCE FOR AN OMEGA-CONOTOXIN, DIHYDROPYRIDINE-INSENSITIVE NEURONAL CA2+ CHANNEL
LUNDY, PM
FREW, R
FULLER, TW
HAMILTON, MG
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1991, 206 (01): : 61 - 68
[8] OMEGA-CONOTOXIN - DIRECT AND PERSISTENT BLOCKADE OF SPECIFIC TYPES OF CALCIUM CHANNELS IN NEURONS BUT NOT MUSCLE
MCCLESKEY, EW
FOX, AP
FELDMAN, DH
CRUZ, LJ
OLIVERA, BM
TSIEN, RW
YOSHIKAMI, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) : 4327 - 4331
[9] P-TYPE CALCIUM CHANNELS BLOCKED BY THE SPIDER TOXIN OMEGA-AGA-IVA
MINTZ, IM
VENEMA, VJ
SWIDEREK, KM
LEE, TD
BEAN, BP
ADAMS, ME
[J]. NATURE, 1992, 355 (6363) : 827 - 829
[10] EVIDENCE FOR MULTIPLE TYPES OF CA2+ CHANNELS IN ACUTELY ISOLATED HIPPOCAMPAL CA3 NEURONS OF THE GUINEA-PIG
MOGUL, DJ
FOX, AP
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1991, 433 : 259 - 281

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