IMPACT OF UV-RADIATION ON THE PRODUCTION PATTERNS AND COMPOSITION OF DISSOLVED FREE AND COMBINED AMINO-ACIDS IN MARINE-PHYTOPLANKTON

被引：34

作者：

GOES, JI

HANDA, N

TAGUCHI, S

HAMA, T

SAITO, H

机构：

[1] HOKKAIDO NATL FISHERIES RES INST, KUSHIRO 085, JAPAN

[2] NATL INST OCEANOG, PANAJI 403004, GOA, INDIA

来源：

JOURNAL OF PLANKTON RESEARCH | 1995年 / 17卷 / 06期

关键词：

D O I：

10.1093/plankt/17.6.1337

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Using the C-13 tracer technique in conjunction with gas chromatographic-mass spectrometric (GC-MS) techniques, we examined the patterns of synthesis and the composition of dissolved free and combined amino acids within phytoplankton photosynthesizing in the presence and absence of natural solar ultraviolet radiation (UVR). At levels that still permitted the uptake of carbon assimilation into the cells, WR caused a marked decline in the overall rate of cart,on incorporated into amino acids and a reduction in the pool size of total cellular amino acids (TCAA). In contrast, absolute concentrations of amino acids within the intracellular dissolved free amino acid (INDFAA) pool (measured using an amino acid analyzer) were higher in the presence of UVR. An examination of the production patterns and composition of amino acids constituting the INDFAA and TCAA pools revealed a marked diminution in the synthesis and accumulation of alanine and valine in the presence of UVR. On the other hand, the rates of synthesis and concentrations of glutamic acid (glutamic acid + glutamine) in the INDFAA and TCAA pools of phytoplankton were higher in samples exposed to UVR. These changes are discussed with reference to the known effects of UVR on nitrogen and carbon assimilation within phytoplankton.

引用

页码：1337 / 1362

页数：26

共 69 条

[41]

Karentz D., Qeaver J.E., Mitchell D.L., Cell survival characteristics and molecular responses of Antartic phytoplankton to UV-B radiation, J. Phycol, 27, pp. 326-341, (1991)

[42]

Keller M.D., Mague T.H., Badenhausenjv L., Glover H.E., Seasonal variations in the production and consumption of amino adds by coastal microplankton, Estuarine Coastal Shelf. Scl, 15, pp. 301-315, (1982)

[43]

Kerr J.B., McElroy C.T., Evidence for large upward trends of ultraviolet-B radiation linked to ozone depletion, Science, 262, pp. 1032-1034, (1993)

[44]

Kouchi H., Direct analysis of 13C abundance in plant carbohydrates by gas chromatography- mass spectrometry, J. Chromatogr., 241, pp. 305-323, (1982)

[45]

Marsot P., Cembella A.D., Colombo J.C., Intracellular and extracellular amino add pools of the marine diatom, Phaeodactylum tncomutum (Bacillariophyceae) grown on unenriched sea water in high cell density dialysis culture, J. Phycol, 27, pp. 478-491, (1991)

[46]

Martin-Jezequel V., Poulet S.A., Harris R.P., Moal J., Samain J.F., Interspecific and intraspedfic composition and variation of free amino adds in marine phytoplankton, Mar Ecol Prog. Ser., 44, pp. 303-313, (1988)

[47]

Miflin B.J., Lea P.J., Amino add metabolism, Annu Rev. Plant Physiol, 28, pp. 299-329, (1977)

[48]

Neale P.J., Lesser M.P., Cullen J.H., Effects of ultraviolet radiation on the photosynthesis of phytoplankton in the vicinity of McMurdo station Antarctica, 62, pp. 125-142, (1994)

[49]

Parsons Maita T.R.Y., Lalli C.M., A Manual of Chemical and Biological Methods for Seawater Analysis, (1984)

[50]

Patrick M.H., Rahn R.O., Photochemistry of DNA and polynudeotides: Products, Photochemistry and Photobiology of Nucleic Acids., 2, pp. 35-95, (1976)

← 1 2 3 4 5 6 7 →