WATER EXCHANGE OF INTERMEDIATES IN A NONHEME IRON, ALPHA-KETOGLUTARATE DIOXYGENASE, DEACETOXY- DEACETYLCEPHALOSPORIN C-SYNTHASE

被引：19

作者：

BALDWIN, JE ^{[1
]}

ADLINGTON, RM ^{[1
]}

CROUCH, NP ^{[1
]}

PEREIRA, IAC ^{[1
]}

APLIN, RT ^{[1
]}

ROBINSON, C ^{[1
]}

机构：

[1] UNIV OXFORD,OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1993年 / 02期

关键词：

D O I：

10.1039/c39930000105

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Incubation of [2-C-13, 3-H-2]penicillin N 1c and [4-H-2]exomethylene cephalosporin C 5b with the enzyme deacetoxy-/deacetylcephalosporin C synthase (DAOC/DAC synthase) in O-18-labelled water gave oxygenated products showing significant incorporation of O-18; the observed levels of incorporation from O-18-labelled water are complementary to the previously reported incorporations which were achieved from molecular oxygen, and we believe this to be a first example of (H2O)-O-18 incorporation into enzymic products catalysed by a non-haem iron, alpha-ketoglutarate dependent dioxygenase, and suggest structures for the exchanging intermediates.

引用

页码：105 / 108

页数：4

共 20 条

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