EPOXIDE FORMATION IN THE ENZYMATIC CONVERSION OF [4-H-2]EXOMETHYLENE CEPHALOSPORIN-C BY DEACETOXYCEPHALOSPORIN DEACETYLCEPHALOSPORIN-C SYNTHASE

被引：10

作者：

BALDWIN, JE ^{[1
]}

ADLINGTON, RM ^{[1
]}

CROUCH, NP ^{[1
]}

PEREIRA, IAC ^{[1
]}

机构：

[1] UNIV OXFORD, OXFORD CTR MOLEC SCI, OXFORD OX1 3QY, ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1992年 / 19期

关键词：

D O I：

10.1039/c39920001448

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The use of a deuterium kinetic isotope effect has enabled the isolation and characterisation of a novel epoxide metabolite produced in the enzymic conversion of [4-H-2]exomethylene cephalosporin C into deacetylcephalosporin C catalysed by the enzyme deacetoxycephalosporin C/deacetylcephalosporin C synthase; V(max)/K(m) experiments are also described, which suggest that the first irreversible event in the conversion of exomethylene cephalosporin C does not involve C-4 hydrogen abstraction.

引用

页码：1448 / 1451

页数：4

共 15 条

[1] INCORPORATION OF H-3 AND C-14 FROM [6-ALPHA-H-3]PENICILLIN-N AND [10-C-14,6-ALPHA-H-3]PENICILLIN-N INTO DEACETOXYCEPHALOSPORIN-C
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[2] STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C
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[3] CEPHALOSPORIN BIOSYNTHESIS - A BRANCHED PATHWAY SENSITIVE TO AN ISOTOPE EFFECT
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[5] HIGH-LEVEL SOLUBLE EXPRESSION AND PURIFICATION OF DEACETOXYCEPHALOSPORIN C/DEACETYLCEPHALOSPORIN C SYNTHASE
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[6] PURIFICATION AND INITIAL CHARACTERIZATION OF AN ENZYME WITH DEACETOXYCEPHALOSPORIN-C SYNTHETASE AND HYDROXYLASE-ACTIVITIES
BALDWIN, JE
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[7] CEPHALOSPORIN-C BIOSYNTHESIS - A BRANCHED PATHWAY SENSITIVE TO A KINETIC ISOTOPE EFFECT
BALDWIN, JE
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CROUCH, NP
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[8] THE ENZYMATIC CONVERSION OF EXOMETHYLENE CEPHALOSPORIN-C INTO DEACETYL CEPHALOSPORIN-C AND THE ROLE OF MOLECULAR-OXYGEN IN CEPHALOSPORIN-C BIOSYNTHESIS
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[10] BALDWIN JE, 1983, J CHEM SOC CHEM COMM, P153

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