ESCHERICHIA-COLI LEADER PEPTIDASE - PRODUCTION OF AN ACTIVE FORM LACKING A REQUIREMENT FOR DETERGENT AND DEVELOPMENT OF PEPTIDE-SUBSTRATES

被引:57
作者
KUO, DW
CHAN, HK
WILSON, CJ
GRIFFIN, PR
WILLIAMS, H
KNIGHT, WB
机构
[1] MERCK SHARP & DOHME LTD,DEPT ENZYMOL,RAHWAY,NJ 07065
[2] MERCK SHARP & DOHME LTD,DEPT BIOCHEM & PATHOL,RAHWAY,NJ 07065
关键词
D O I
10.1006/abbi.1993.1283
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The report by Zimmerman et al. (J. Biol. Chem. 257, 1982, 6529-6536) that the active site of Escherichia coli leader peptidase (LPase) is located in the periplasm led us to explore the possibility that soluble, active short forms of LPase could be produced. Detergent free Δ2-75 mutant protein (LPase-sf) lacking the two N-terminal transmembrane spanning and the cytoplasmic domains was produced in high yield. The mass of the protein determined by electrospray ionisation mass spectrometry was 27,952 amu. The increase of 42 amu over that predicted by the expected amino acid sequence indicates that the N-terminus of LPase-sf is acetylated. This is consistent with the inability to obtain an N-terminal sequence. LPase-sf lacks the site of autolysis present in LPase, thus circumventing problems associated with enzyme autocatalytic instability. LPase-sf and wild type LPase displayed comparable activity versus two peptide substrates. The peptides, based upon the cleavage sites of procoat and the precursor of maltose binding protein, were processed at the expected sites. In addition, the activity of both LPase′s was not inhibited by classical inhibitors of the four classes of proteases. LPase-sf displayed similar activity in the presence and absence of detergent. Wild type LPase displayed specificity for alanine in the P1 subsite of the peptide WSASALX*KI and did not hydrolyse peptides with glycine, valine, or serine in that position. The availability of a detergent-free active form of LPase should facilitate mechanistic and structural studies. © 1993 Academic Press, Inc.
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页码:274 / 280
页数:7
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