A COSMID LIBRARY SPECIFIC FOR HUMAN-CHROMOSOME REGIONS 6P21.3 AND 6Q27

被引：10

作者：

SHORTLE, VP

MALASPINA, P

FOX, M

DOOLEY, JS

VOLZ, A

ZIEGLER, A

TROWSDALE, J

MORRISON, KM

EDWARDS, YH

机构：

[1] MRC,HUMAN BIOCHEM GENET UNIT,LONDON NW1 2HE,ENGLAND

[2] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,INST EXPTL ONKOL & TRANSPLANTAT MED,W-1000 BERLIN 19,GERMANY

[3] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND

来源：

MAMMALIAN GENOME | 1993年 / 4卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1007/BF00364783

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have explored the potential of irradiation-fusion gene transfer (IFGT) hybrids as a source of well-defined human chromosome fragments from which probes can be derived. Extensive characterization of the IFGT hybrid 4J4 with a full panel of markers from Chromosome (Chr) 6 showed that the human DNA content derives largely from 6p21.3 and 6q27. A cosmid library has been constructed from 4J4 DNA, and 370 recombinants containing human DNA have been isolated and overlapping clones ordered into 20 contigs. Regional localization of representative clones from each contig, determined by fluorescent in situ hybridization (FISH), places 13 contigs in 6q27 and 6 in 6p21.3. Preliminary screening of cDNA libraries with selected cosmids has identified two expressed sequences. Since there are a number of medically important genes in both these regions of human Chr 6 with several disease loci linked to the HLA-A region in 6p21.3 and various tumor suppressor genes to 6q27, this library will provide a valuable resource to aid the isolation of candidate genes for these diseases. In addition, unique markers for detailed physical and genetic mapping of these regions of human Chr 6 can be easily obtained.

引用

页码：493 / 498

页数：6

共 21 条

[1] SEQUENCE IDENTIFICATION OF 2,375 HUMAN BRAIN GENES [J].

ADAMS, MD ;

DUBNICK, M ;

KERLAVAGE, AR ;

MORENO, R ;

KELLEY, JM ;

UTTERBACK, TR ;

NAGLE, JW ;

FIELDS, C ;

VENTER, JC .

NATURE, 1992, 355 (6361) :632-634

[2] IDENTIFICATION OF HUMAN GENOMIC CLONES CODING THE MAJOR HISTOCOMPATIBILITY ANTIGENS HLA-A2 AND HLA-B7 BY DNA-MEDIATED GENE-TRANSFER [J].

BARBOSA, JA ;

KAMARCK, ME ;

BIRO, PA ;

WEISSMAN, SM ;

RUDDLE, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (20) :6327-6331

[3] RADIATION HYBRID MAPPING [J].

COX, DR .

CYTOGENETICS AND CELL GENETICS, 1992, 59 (2-3) :80-81

[4]

ELKAHLOUN A, 1993, HUM MOL GENET, V2, P55

[5] DELETIONS INVOLVING 2 DISTINCT REGIONS OF 6Q IN B-CELL NON-HODGKIN LYMPHOMA [J].

GAIDANO, G ;

HAUPTSCHEIN, RS ;

PARSA, NZ ;

OFFIT, K ;

RAO, PH ;

LENOIR, G ;

KNOWLES, DM ;

CHAGANTI, RSK ;

DALLAFAVERA, R .

BLOOD, 1992, 80 (07) :1781-1787

[6]

GAIDANO G, 1993, IN PRESS HUM MOL GEN

[7] A PANEL OF IRRADIATION-REDUCED HYBRIDS SELECTIVELY RETAINING HUMAN CHROMOSOME-11P13 - THEIR STRUCTURE AND USE TO PURIFY THE WAGR GENE-COMPLEX [J].

GLASER, T ;

ROSE, E ;

MORSE, H ;

HOUSMAN, D ;

JONES, C .

GENOMICS, 1990, 6 (01) :48-64

[8]

HEINKOFF S, 1987, METHOD ENZYMOL, V155, P156

[9] ISOLATION OF HLA LOCUS-SPECIFIC DNA PROBES FROM THE 3'-UNTRANSLATED REGION [J].

KOLLER, BH ;

SIDWELL, B ;

DEMARS, R ;

ORR, HT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (16) :5175-5178

[10]

KRAVITZ K, 1979, AM J HUM GENET, V31, P601

← 1 2 3 →