DIFFERENTIAL EFFECTS OF OUABAIN ON HOST-SPECIFIED AND SINDBIS VIRUS-SPECIFIED PROTEIN-SYNTHESIS

被引：23

作者：

GARRY, RF ^{[1
]}

WESTBROOK, K ^{[1
]}

WAITE, MRF ^{[1
]}

机构：

[1] UNIV TEXAS,DEPT MICROBIOL,AUSTIN,TX 78712

来源：

VIROLOGY | 1979年 / 99卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0042-6822(79)90051-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ouabain, a specific inhibitor of the membrane-associated Na+/K+-ATPase (sodium pump), has a differential effect on protein synthesis in Sindbis virus-infected and uninfected chick embryo fibroblast (CEF) cultures. At low concentrations, cell-specified protein synthesis is inhibited while Sindbis virus-directed protein synthesis is stimulated. At higher ouabain concentrations, which inhibit protein synthesis in uninfected CEF cells 95% or more, viral protein synthesis continues. Ouabain treatment of uninfected CEF cells raises the intracellular Na+ concentration and lowers the intracellular K+ concentration. The effect of Sindbis virus infection is similar. The changes in intracellular Na+ and K+ concentrations appear responsible for the selective inhibition of the translation of host cell mRNAs in Sindbis virus-infected or ouabain-treated CEF. © 1979.

引用

页码：179 / 182

页数：4

共 6 条

[1]

DAHL JL, 1974, ANNU REV BIOCHEM, V43, P327

[2] NA+ AND K+ CONCENTRATIONS AND THE REGULATION OF THE INTERFERON SYSTEM IN CHICK-CELLS [J].