HUMAN BRADYKININ B2-RECEPTOR - NUCLEOTIDE-SEQUENCE ANALYSIS AND ASSIGNMENT TO CHROMOSOME-14

被引：55

作者：

POWELL, SJ

SLYNN, G

THOMAS, C

HOPKINS, B

BRIGGS, I

GRAHAM, A

机构：

[1] ICI PHARMACEUT PLC,DEPT BIOTECHNOL,ALDERLEY PK,MACCLESFIELD SK10 4TG,CHESHIRE,ENGLAND

[2] ICI PHARMACEUT PLC,DEPT BIOSCI 2,MACCLESFIELD SK10 4TG,CHESHIRE,ENGLAND

来源：

GENOMICS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1006/geno.1993.1084

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Functional cDNA clones for human bradykinin B2 receptor were isolated from uterus RNA by a polymerase chain reaction (PCR)-based method and by screening a human cosmid library with rat bradykinin B2 receptor probe. We isolated several overlapping clones from the cosmid library, each of which encodes the en-ire protein coding sequence. The human bradykinin B2 receptor gene codes for a 364-amino-acid protein with a molecular mass of 41,442 Da that is highly homologous to rat bradykinin B2 receptor cDNA (81%). The entire human cDNA sequence was cloned into an expression vector and mRNA was synthesised by in vitro transcription. Applications of bradykinin caused membrane current responses in Xenopus oocytes injected with the in vitro-synthesized mRNA. Preincubation with the potent B2 antagonist, HOE140, prevented this response. The genomic clone is intronless, and we have identified an upstream promoter region and a downstream polyadenylation signal. The human bradykinin B2 receptor gene has been mapped to chromosome 14 using PCR to specifically amplify DNA from somatic cell hybrids. © 1993 by Academic Press, Inc.

引用

页码：435 / 438

页数：4

共 10 条

[1]

FROHMAN MA, 1989, TECHNIQUE, V1, P165

[2]

FROHMAN MA, 1988, P NATL ACAD SCI USA, V85, P8898

[3]

GRAHAM A, 1991, HUM GENET, V86, P509

[4] ISOLATION AND CHARACTERIZATION OF THE HUMAN LUNG NK-2 RECEPTOR GENE USING RAPID AMPLIFICATION OF CDNA ENDS [J].

GRAHAM, A ;

HOPKINS, B ;

POWELL, SJ ;

DANKS, P ;

BRIGGS, I .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 177 (01) :8-16

[5]

MAHAN LC, 1990, MOL PHARMACOL, V37, P785

[6] EXPRESSION CLONING OF A RAT B2-BRADYKININ RECEPTOR [J].

MCEACHERN, AE ;

SHELTON, ER ;

BHAKTA, S ;

OBERNOLTE, R ;

BACH, C ;

ZUPPAN, P ;

FUJISAKI, J ;

ALDRICH, RW ;

JARNAGIN, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7724-7728

[7] KININ FORMATION - MECHANISMS AND ROLE IN INFLAMMATORY DISORDERS [J].

PROUD, D ;

KAPLAN, AP .

ANNUAL REVIEW OF IMMUNOLOGY, 1988, 6 :49-83

[8]

REGOLI D, 1980, PHARMACOL REV, V32, P1

[9] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].

SAIKI, RK ;

SCHARF, S ;

FALOONA, F ;

MULLIS, KB ;

HORN, GT ;

ERLICH, HA ;

ARNHEIM, N .

SCIENCE, 1985, 230 (4732) :1350-1354

[10]

SCHROEDER WT, 1985, AM J HUM GENET, V37, P868

← 1 →