PYRUVATE-DEHYDROGENASE DEPHOSPHORYLATION IN RAT-BRAIN SYNAPTOSOMES AND MITOCHONDRIA - EVIDENCE FOR A CALCIUM-MEDIATED EFFECT IN RESPONSE TO DEPOLARIZATION, AND VARIATIONS DUE TO AGING

被引：15

作者：

BOGONEZ, E ^{[1
]}

GOMEZPUERTAS, P ^{[1
]}

SATRUSTEGUI, J ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,CSIC,CTR BIOL,DEPT BIOL MOLEC,E-28049 MADRID,SPAIN

来源：

NEUROSCIENCE LETTERS | 1992年 / 142卷 / 02期

关键词：

SYNAPTOSOME; MITOCHONDRION; PYRUVATE DEHYDROGENASE; DEPHOSPHORYLATION; CALCIUM; AGING;

D O I：

10.1016/0304-3940(92)90354-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The phosphorylation state of P42, the phosphorylated, catalytically inactive, alpha-subunit of pyruvate dehydrogenase (PDH), decreased markedly (42.4%) in response to K+-depolarization of synaptosomes. The dephosphorylation was rapid (5-15 s), calcium-dependent and could also be observed in isolated mitochondria exposed to a rise in extramitochondrial calcium, suggesting that P42 dephosphorylation may act as a calcium sensor in the mitochondrial matrix. The depolarization-dependent dephosphorylation rate of P42 was decreased in synaptosomes derived from 24-month-old animals with respect to 3-month-old adults. The relevance of these results in terms of PDH activation during ageing is discussed.

引用

页码：123 / 127

页数：5

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