SYNAPTIC TARGETING DOMAINS OF SYNAPSIN-I REVEALED BY TRANSGENIC EXPRESSION IN PHOTORECEPTOR CELLS

被引:31
作者
GEPPERT, M
ULLRICH, B
GREEN, DG
TAKEI, K
DANIELS, L
DECAMILLI, P
SUDHOF, TC
HAMMER, RE
机构
[1] UNIV TEXAS, SW MED CTR, DEPT MOLEC GENET, DALLAS, TX 75235 USA
[2] UNIV TEXAS, SW MED CTR, DEPT BIOCHEM, DALLAS, TX 75235 USA
[3] UNIV TEXAS, SW MED CTR, HOWARD HUGHES MED INST, DALLAS, TX 75235 USA
[4] UNIV MICHIGAN, DEPT OPHTHALMOL, ANN ARBOR, MI 48104 USA
[5] YALE UNIV, SCH MED, DEPT CELL BIOL, NEW HAVEN, CT 06510 USA
[6] YALE UNIV, SCH MED, HOWARD HUGHES MED INST, NEW HAVEN, CT 06510 USA
关键词
PHOTORECEPTOR CELLS; SYNAPSES; SYNAPSINS; TRANSGENIC EXPRESSION;
D O I
10.1002/j.1460-2075.1994.tb06681.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Synapsins are abundant nerve terminal proteins present at all synapses except for ribbon synapses, e.g. photoreceptor cell synapses. Multiple functions have been proposed for synapsins, including clustering of synaptic vesicles and regulation of synaptic vesicle exocytosis. To investigate the physiological functions of synapsin and to ascertain which domains of synapsin are involved in synaptic targeting in vivo, we expressed synapsin Ib and its N- and C-terminal domains in the photoreceptor cells of transgenic mice. In these cells synapsin Ib is targeted efficiently to synaptic vesicles but has no significant effect on the development, structure or physiology of the synapses. This suggests that synapsin I does not have dominant physiological or morphoregulatory functions at these synapses. Full-length synapsin Ib and the N-terminal domains of synapsin Ib but not its C-terminal domains are transported to synapses, revealing that the molecular apparatus for synaptic targeting of synapsins is also present in cells which form ribbon synapses that normally lack synapsins. This apparatus appears to utilize the conserved N-terminal domains that are shared between all synapsins.
引用
收藏
页码:3720 / 3727
页数:8
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