COMPARISON OF THE STRATEGIES OF EXPRESSION OF 5 TYMOVIRUS RNAS BY INVITRO TRANSLATION STUDIES

被引：11

作者：

KADARE, G ^{[1
]}

DRUGEON, G ^{[1
]}

SAVITHRI, HS ^{[1
]}

HAENNI, AL ^{[1
]}

机构：

[1] UNIV PARIS 07, CNRS, INST JACQUES MONOD, 2 PL JUSSIEU, TOUR 43, F-75251 PARIS 05, FRANCE

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-2-493

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Total nucleotide sequencing of the RNA genome of various tymoviruses has demonstrated that the overall genome organization of these viruses is identical. Furthermore, the strategies of expression of the turnip yellow mosaic virus (TYMV) genome have been established by in vitro translation studies; these include the synthesis of a subgenomic RNA, the utilization of overlapping open reading frames (ORFs) and maturation of a polyprotein. In the experiments described here, the strategies of expression of other tymovirus (eggplant mosaic virus, ononis yellow mosaic virus, belladonna mottle virus and physalis mottle virus) genomes have been compared to those used by the TYMV genome, in particular to determine whether these tymoviruses also resort to the expression of overlapping ORFs and maturation of a polyprotein.

引用

页码：493 / 498

页数：6