学术探索
学术期刊
新闻热点
数据分析
智能评审

PHOSPHORYLATION OF THE PHOSPHATASE MODULATOR SUBUNIT (INHIBITOR-2) BY CASEIN KINASE-1 - IDENTIFICATION OF THE PHOSPHORYLATION SITES

被引：25
作者：
AGOSTINIS, P
MARIN, O
JAMES, P
HENDRIX, P
MERLEVEDE, W
VANDENHEEDE, JR
PINNA, LA
机构：
[1] CATHOLIC UNIV LEUVEN,FAC GENEESKUNDE,AFDELING BIOCHEM,CAMPUS GASTHUISBERG,B-3000 LOUVAIN,BELGIUM
[2] UNIV PADUA,DIPARTIMENTO CHIM BIOL,I-35100 PADUA,ITALY
[3] SWISS FED INST TECHNOL,PROT CHEM SERV,CH-8092 ZURICH,SWITZERLAND
[4] SWISS FED INST TECHNOL,FORSCHUNGSLAB,CH-8092 ZURICH,SWITZERLAND
来源：
FEBS LETTERS | 1992年 / 305卷 / 02期
关键词：
PHOSPHATASE MODULATOR; INHIBITOR-2; CASEIN KINASE-1; CASEIN KINASE-2; PHOSPHORYLATION SITE;
D O I：
10.1016/0014-5793(92)80877-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The isolated modulator subunit of the inactive protein phosphatase-1 is phosphorylated in vitro by casein kinase-1 at two different sites: Ser-86 and Ser-174. The Ser-86 site is a common target for casein kinase-1 and casein kinase-2, but is preferentially phosphorylated by the former enzyme. The Ser-174 site seems to be specific for casein kinase-1, and is phosphorylated at a slower rate. These results give a new insight into the in vitro phosphorylation pattern of the modulator subunit of the phosphatase and provides additional data on the specificity of casein kinase-1.
引用
收藏
页码：121 / 124
页数：4
相关论文
共 21 条
[11]   ANALYSIS OF THE INVIVO PHOSPHORYLATION STATE OF PROTEIN PHOSPHATASE INHIBITOR-2 FROM RABBIT SKELETAL-MUSCLE BY FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY [J].
HOLMES, CFB ;
TONKS, NK ;
MAJOR, H ;
COHEN, P .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 929 (02) :208-219
[12]   IDENTIFICATION OF THE SITES ON RABBIT SKELETAL-MUSCLE PROTEIN PHOSPHATASE INHIBITOR-2 PHOSPHORYLATED BY CASEIN KINASE-II [J].
HOLMES, CFB ;
KURET, J ;
CHISHOLM, AAK ;
COHEN, P .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 870 (03) :408-416
[13]   SUBSTRATE-SPECIFICITY OF CYCLIC AMP-DEPENDENT PROTEIN KINASE [J].
KEMP, BE ;
BYLUND, DB ;
HUANG, TS ;
KREBS, EG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) :3448-3452
[14]   A SYNTHETIC BETA-CASEIN PHOSPHOPEPTIDE AND ANALOGS AS MODEL SUBSTRATES FOR CASEIN KINASE-1, A UBIQUITOUS, PHOSPHATE DIRECTED PROTEIN-KINASE [J].
MEGGIO, F ;
PERICH, JW ;
REYNOLDS, EC ;
PINNA, LA .
FEBS LETTERS, 1991, 283 (02) :303-306
[15]  
MEGGIO F, 1981, J BIOL CHEM, V256, P1958
[16]   THE COMPARATIVE EFFICIENCIES OF THE SER(P)-RESIDUE, THR(P)-RESIDUE AND TYR(P)-RESIDUES AS SPECIFICITY DETERMINANTS FOR CASEIN KINASE-1 [J].
MEGGIO, F ;
PERICH, JW ;
MARIN, O ;
PINNA, LA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 182 (03) :1460-1465
[17]   CASEIN KINASE-2 - AN EMINENCE-GRISE IN CELLULAR-REGULATION [J].
PINNA, LA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1054 (03) :267-284
[18]   PURIFICATION OF CASEIN KINASE-I AND ISOLATION OF CDNAS ENCODING MULTIPLE CASEIN KINASE-I-LIKE ENZYMES [J].
ROWLES, J ;
SLAUGHTER, C ;
MOOMAW, C ;
HSU, J ;
COBB, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9548-9552
[19]  
TUAZON PT, 1991, ADV 2ND MESSENGER PH, V23, P133
[20]  
VANDENHEEDE JR, 1991, CELLULAR REGULATION, P321
123