DEVELOPMENT OF A RANDOM AMPLIFICATION OF POLYMORPHIC DNA TYPING METHOD FOR LISTERIA-MONOCYTOGENES

被引：80

作者：

LAWRENCE, LM ^{[1
]}

HARVEY, J ^{[1
]}

GILMOUR, A ^{[1
]}

机构：

[1] DEPT AGR NO IRELAND,CTR AGR & FOOD SCI,BELFAST BT9 5PX,NORTH IRELAND

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 09期

关键词：

D O I：

10.1128/AEM.59.9.3117-3119.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 10-mer primer OPM-01 (5'-GTT GGT GGC T-3') was used to generate random amplification of polymorphic DNA (RAPD) profiles by polymerase chain reaction for 91 strains of Listeria monocytogenes from raw milk, food, and veterinary, medical, and food-environmental sources. The profiles obtained contained 1 to 10 bands within the molecular size range of 0.5 to 5.0 kbp. Reproducibility was enhanced by annealing at low stringency and introducing a 1-min ramp time between annealing and extension temperatures. Thirty-three RAPD profiles were observed, with specific profiles being observed for strains from each source. RAPD profiles allowed discrimination within serogroups, although five RAPD profiles which were not confined to one serotype were found. Within food strains, one RAPD profile was more common than others, suggesting this to be a common type among strains from this source.

引用

页码：3117 / 3119

页数：3

共 12 条

[1]

BASSAM BJ, 1992, APPL MICROBIOL BIOT, V38, P70, DOI 10.1007/BF00169422

[2] ARBITRARY PRIMER POLYMERASE CHAIN-REACTION, A POWERFUL METHOD TO IDENTIFY BACILLUS-THURINGIENSIS SEROVARS AND STRAINS [J].

BROUSSEAU, R ;

SAINTONGE, A ;

PREFONTAINE, G ;

MASSON, L ;

CABANA, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (01) :114-119

[3] DNA AMPLIFICATION FINGERPRINTING USING VERY SHORT ARBITRARY OLIGONUCLEOTIDE PRIMERS [J].

CAETANOANOLLES, G ;

BASSAM, BJ ;

GRESSHOFF, PM .

BIO-TECHNOLOGY, 1991, 9 (06) :553-557

[4] DIFFERENTIATION OF LISTERIA-MONOCYTOGENES AND LISTERIA-INNOCUA BY 16S RIBOSOMAL-RNA GENES AND INTRASPECIES DISCRIMINATION OF LISTERIA-MONOCYTOGENES STRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMORPHISMS [J].

CZAJKA, J ;

BSAT, N ;

PIANI, M ;

RUSS, W ;

SULTANA, K ;

WIEDMANN, M ;

WHITAKER, R ;

BATT, CA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (01) :304-308

[5] OCCURRENCE OF LISTERIA SPECIES IN RAW-MILK AND DAIRY-PRODUCTS PRODUCED IN NORTHERN-IRELAND [J].

HARVEY, J ;

GILMOUR, A .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (02) :119-125

[6]

HARVEY J, IN PRESS INT J FOOD

[7] RAPID ANALYSIS OF CAMPYLOBACTER ISOLATES - DNA FINGERPRINTING WITHOUT THE NEED TO PURIFY DNA [J].

MAZURIER, S ;

VANDEGIESSEN, A ;

HEUVELMAN, K ;

WERNARS, K .

LETTERS IN APPLIED MICROBIOLOGY, 1992, 14 (06) :260-262

[8] A COMPARATIVE-STUDY OF RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS AND CONVENTIONAL PHAGE TYPING FOR EPIDEMIOLOGIC STUDIES OF LISTERIA-MONOCYTOGENES ISOLATES [J].

MAZURIER, SI ;

AUDURIER, A ;

MARQUETVANDERMEE, N ;

NOTERMANS, S ;

WERNARS, K .

RESEARCH IN MICROBIOLOGY, 1992, 143 (05) :507-512

[9] TYPING OF LISTERIA STRAINS BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA [J].

MAZURIER, SI ;

WERNARS, K .

RESEARCH IN MICROBIOLOGY, 1992, 143 (05) :499-505

[10]

Seeliger H, 1979, METHOD MICROBIOL, V13, P31

← 1 2 →