IDENTIFICATION OF 4 NEW PROKARYOTIC BACTERIOFERRITINS, FROM HELICOBACTER-PYLORI, ANABAENA-VARIABILIS, BACILLUS-SUBTILIS AND TREPONEMA-PALLIDUM, BY ANALYSIS OF GENE-SEQUENCES

被引：75

作者：

EVANS, DJ ^{[1
]}

EVANS, DG ^{[1
]}

LAMPERT, HC ^{[1
]}

NAKANO, H ^{[1
]}

机构：

[1] BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030

来源：

GENE | 1995年 / 153卷 / 01期

基金：

美国国家卫生研究院;

关键词：

CYTOCHROME; FERRITIN; GASTRITIS; HEME; IRON; MULTIFUNCTIONAL; NEUTROPHIL-ACTIVATING PROTEIN;

D O I：

10.1016/0378-1119(94)00774-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide (nt) sequence of the Helicobacter pylori (Hp) napA gene, encoding neutrophil-activating protein A (HPNAP) was determined. Alignment of this sequence with those of known bacterioferritins (Bfr) revealed sequence homology and conservation of a 7-amino-acid (aa) motif constituting the ferroxidase (Frx) center of Bfr in the HPNAP. The N-terminal aa sequence deduced from the iron-regulated mrgC gene of Bacillus subtilis [Chen et al., J. Bacteriol. 175 (1993) 5428-5437] is highly similar to that of HPNAP and contains five Frx center aa residues. The deduced aa sequences for proteins of unknown function in Treponema pallidum [Walfield et al., Infect. Immun. 57 (1989) 633-635] and in the cyanobacterium Anabaena variabilis [Sato, GenBank accession No. JU0384 (1991)] identify these two proteins as Bfr. Although the DNA-binding protein from starved cells of Escherichia coli [Almiron et al., Genes Dev. 6 (1992) 2646-2654] is clearly a HPNAP/Bfr homologue, a significant part of its Frx center is missing. It is unlikely that the intracellular function of HPNAP is related to its ability to activate neutrophils.

引用

页码：123 / 127

页数：5

共 30 条

[1] DNA DIVERSITY AMONG CLINICAL ISOLATES OF HELICOBACTER-PYLORI DETECTED BY PCR-BASED RAPD FINGERPRINTING [J].