GENERAL SPLICING FACTOR SF2 ASF PROMOTES ALTERNATIVE SPLICING BY BINDING TO AN EXONIC SPLICING ENHANCER

被引：265

作者：

SUN, Q

MAYEDA, A

HAMPSON, RK

KRAINER, AR

ROTTMAN, FM

机构：

[1] CASE WESTERN RESERVE UNIV,DEPT MOLEC BIOL & MICROBIOL,CLEVELAND,OH 44106

[2] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724

来源：

GENES & DEVELOPMENT | 1993年 / 7卷 / 12B期

关键词：

ALTERNATIVE SPLICING; BOVINE GROWTH HORMONE PREMESSENGER RNA; SF2; ASF; HNRNP-A1; EXONIC SPLICING ENHANCER;

D O I：

10.1101/gad.7.12b.2598

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

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页码：2598 / 2608

页数：11

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