BATCH AND FED BATCH CULTIVATIONS FOR THE TEMPERATURE INDUCED PRODUCTION OF A RECOMBINANT PROTEIN IN ESCHERICHIA-COLI

被引：40

作者：

STRANDBERG, L

ENFORS, SO

机构：

[1] Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 08期

关键词：

D O I：

10.1007/BF01033419

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The temperature induced intracellular production of the fused protein SpA-beta-gal (protein A/beta-galactosidase) with E. coli was compared in batch and fed batch culture. By introducing fed batch cultivation a final cell dry weight of 77.0 g/l was achieved, as compared to 16.4 g/l in batch cultivation. The concentration of SpA-beta-gal in the fed batch cultivation was very high, 19.2 g/l. This corresponded to 25% of the cell dry weight.

引用

收藏

页码：609 / 614

页数：6

相关论文

共 19 条

[1] EFFECTS OF PLASMID AMPLIFICATION AND RECOMBINANT GENE-EXPRESSION ON THE GROWTH-KINETICS OF RECOMBINANT ESCHERICHIA-COLI [J].

BETENBAUGH, MJ ;

BEATY, C ;

DHURJATI, P .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (11) :1425-1436

[2] PRODUCTION OF HUMAN ALPHA-CONSENSUS INTERFERON IN RECOMBINANT ESCHERICHIA-COLI [J].

FIESCHKO, J ;

RITCH, T .

CHEMICAL ENGINEERING COMMUNICATIONS, 1986, 45 (1-6) :229-240

[3] CONTROL OF IMMOBILIZED, NON-GROWING CELLS FOR CONTINUOUS PRODUCTION OF METABOLITES [J].

FORBERG, C ;

ENFORS, SO ;

HAGGSTROM, L .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1983, 17 (03) :143-147

[4] DIFFERENT APPROACHES TO STABILIZE A RECOMBINANT FUSION PROTEIN [J].

HELLEBUST, H ;

MURBY, M ;

ABRAHMSEN, L ;

UHLEN, M ;

ENFORS, SO .

BIO-TECHNOLOGY, 1989, 7 (02) :165-168

[5]

HOLME T, 1970, Process Biochemistry, V5, P62

[6] PRODUCTION OF RECOMBINANT HUMAN GROWTH-HORMONE IN ESCHERICHIA-COLI - EXPRESSION OF DIFFERENT PRECURSORS AND PHYSIOLOGICAL-EFFECTS OF GLUCOSE, ACETATE, AND SALTS [J].

JENSEN, EB ;

CARLSEN, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 36 (01) :1-11

[7] HIGH-CELL DENSITY FERMENTATION STUDIES OF RECOMBINANT ESCHERICHIA-COLI STRAINS EXPRESSING HUMAN INTERLEUKIN-1-BETA [J].

JUNG, G ;

DENEFLE, P ;

BECQUART, J ;

MAYAUX, JF .

ANNALES DE L INSTITUT PASTEUR-MICROBIOLOGIE, 1988, 139 (01) :129-146

[8] FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION OF THE TAC-PROMOTER-CONTROLLED CALF PROCHYMOSIN CDNA IN ESCHERICHIA-COLI HB101 [J].

KAPRALEK, F ;

JECMEN, P ;

SEDLACEK, J ;

FABRY, M ;

ZADRAZIL, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (01) :71-79

[9] COMPARISON OF GROWTH, ACETATE PRODUCTION, AND ACETATE INHIBITION OF ESCHERICHIA-COLI STRAINS IN BATCH AND FED-BATCH FERMENTATIONS [J].

LULI, GW ;

STROHL, WR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (04) :1004-1011

[10] SIMPLE CONSTRAINED-OPTIMIZATION VIEW OF ACETATE OVERFLOW IN ESCHERICHIA-COLI [J].

MAJEWSKI, RA ;

DOMACH, MM .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (07) :732-738