BATCH AND FED BATCH CULTIVATIONS FOR THE TEMPERATURE INDUCED PRODUCTION OF A RECOMBINANT PROTEIN IN ESCHERICHIA-COLI

被引：40

作者：

STRANDBERG, L

ENFORS, SO

机构：

[1] Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 08期

关键词：

D O I：

10.1007/BF01033419

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The temperature induced intracellular production of the fused protein SpA-beta-gal (protein A/beta-galactosidase) with E. coli was compared in batch and fed batch culture. By introducing fed batch cultivation a final cell dry weight of 77.0 g/l was achieved, as compared to 16.4 g/l in batch cultivation. The concentration of SpA-beta-gal in the fed batch cultivation was very high, 19.2 g/l. This corresponded to 25% of the cell dry weight.

引用

页码：609 / 614

页数：6

共 19 条

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