THE EFFECT OF RECOVERY CONDITIONS ON THE APPARENT HEAT-RESISTANCE OF BACILLUS-CEREUS SPORES

被引：29

作者：

GONZALEZ, I ^{[1
]}

LOPEZ, M ^{[1
]}

MAZAS, M ^{[1
]}

GONZALEZ, J ^{[1
]}

BERNARDO, A ^{[1
]}

机构：

[1] UNIV LEON,FAC VET,E-24071 LEON,SPAIN

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 78卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1995.tb03098.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of recovery media and incubation temperature on the apparent heat resistance of three ATCC strains (4342, 7004 and 9818) of Bacillus cereus spores were studied. Nutrient Agar (NA), Tryptic Soy Agar (TSA), Plate Count Agar (PCA) and Milk Agar (MA) as the media and temperatures in the range of 15-40 degrees C were used to recover heated spores. Higher counts of heat injured spores were obtained on PCA and NA. The optimum subculture temperature was about 5 degrees C below the optimum temperature for unheated spores. No significant differences in heat resistance were observed with the different recovery conditions except for strains 4342 and 9818 when MA was used as plating medium. Large differences in D-values were found among the strains (D-100 = 0.28 min for 7004; D-100 = 0.99 min for 4342; D-100 = 4.57 min for 9818). The 7004 strain showed a sub-population with a greater heat resistance. The z values obtained for the three strains studied under the different recovery conditions were similar (7.64 degrees C + 0.25).

引用

页码：548 / 554

页数：7

共 30 条

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