CLONING OF CDNA AND GENOMIC DNA FOR HUMAN CYTOCHROME-P-45011-BETA

被引:94
作者
KAWAMOTO, T
MITSUUCHI, Y
TODA, K
MIYAHARA, K
YOKOYAMA, Y
NAKAO, K
HOSODA, K
YAMAMOTO, Y
IMURA, H
SHIZUTA, Y
机构
[1] KOCHI MED SCH,DEPT MED CHEM,NANKO KU,KOCHI 783,JAPAN
[2] KYOTO UNIV,FAC MED,DEPT MED,SAKYO KU,KYOTO 606,JAPAN
[3] KOCHI MED SCH,DEPT INTERNAL MED,NANKO KU,KOCHI 783,JAPAN
关键词
Adrenal tumor; cDNA; Genomic DNA; Monooxygenase; P-450[!sub]11β[!/sub; Steroidogenesis;
D O I
10.1016/0014-5793(90)81190-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A full-length cDNA clone encoding steroid 11β-hydroxylase (P-45011β) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-45011β gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-45011β gene. © 1990.
引用
收藏
页码:345 / 349
页数:5
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