METABOLISM OF BENZO[A]PYRENE AND PERSISTENCE OF DNA ADDUCTS IN THE BROWN BULLHEAD (ICTALURUS-NEBULOSUS)

被引：8

作者：

SIKKA, HC ^{[1
]}

STEWARD, AR ^{[1
]}

KANDASWAMI, C ^{[1
]}

RUTKOWSKI, JP ^{[1
]}

ZALESKI, J ^{[1
]}

KUMAR, S ^{[1
]}

EARLEY, K ^{[1
]}

GUPTA, RC ^{[1
]}

机构：

[1] UNIV KENTUCKY,MED CTR,DEPT PREVENT MED ENVIRONM HLTH,LEXINGTON,KY 40536

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1991年 / 100卷 / 1-2期

关键词：

D O I：

10.1016/0742-8413(91)90115-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. The in vitro metabolism of [H-3]benzo[a]pyrene (BP) and [C-14]benzo[a]pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. 2. Compared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of BP-7,8-diol (predominantly the [-] enantiomer) and smaller amounts of BP-4,5-diol. 3. BP phase I metabolites were efficiently converted by freshly isolated bullhead hepatocytes to conjugates, predominantly glucuronides. 4. BP-7,8-diol was metabolized by hepatocytes 4-fold more rapidly than was BP and was converted to approximately equal amounts of glucuronides, glutathione conjugates and sulfates. 5. BP-DNA adducts formed in bullhead liver with a lag time of several days and maximum adduct formation at 25-30 days. The major adduct was anti-BDPE-deoxyguanosine.

引用

页码：25 / 28

页数：4

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