CHROMOSOMAL STRUCTURE AND EXPRESSION OF THE HUMAN OTF1 LOCUS ENCODING THE OCT-1 PROTEIN

被引：31

作者：

STURM, RA

CASSADY, JL

DAS, G

ROMO, A

EVANS, GA

机构：

[1] SALK INST BIOL STUDIES, MOLEC GENET LAB, LA JOLLA, CA 92037 USA

[2] COLD SPRING HARBOR LAB, COLD SPRING HARBOR, NY 11724 USA

来源：

GENOMICS | 1993年 / 16卷 / 02期

关键词：

D O I：

10.1006/geno.1993.1194

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genomic structure of the POU domain containing oct-1 gene (OTF1 locus) coding region has been determined using human DNA recombinant bacteriophage and a yeast artificial chromosome clone. The gene is encoded by 16 exons spanning over 150 kb, and the Oct-1 protein reading frame has been extended to 766 amino acids. The exonic structure has been compared to the mouse Oct-2 protein and reveals a conservation of exon-intron boundaries as well as protein sequence similarity. To provide insight into Oct-1 control of transcriptional regulation during the cell-cycle the expression of the oct -1 gene was examined during cellular DNA replication and shows that the steady-state level of the oct-1 mRNA is not S-phase regulated. © 1993 Academic Press, Inc.

引用

页码：333 / 341

页数：9

共 62 条

[1] Ausubel F, 1988, CURRENT PROTOCOLS MO
[2] BURKE DT, 1991, METHOD ENZYMOL, V194, P251
[3] EUKARYOTIC START AND STOP TRANSLATION SITES
CAVENER, DR
RAY, SC
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (12) : 3185 - 3192
[4] CHEN R, 1990, NATURE, V436, P583
[5] THE B-CELL-SPECIFIC OCT-2 PROTEIN CONTAINS POU BOX-TYPE AND HOMEO BOX-TYPE DOMAINS
CLERC, RG
CORCORAN, LM
LEBOWITZ, JH
BALTIMORE, D
SHARP, PA
[J]. GENES & DEVELOPMENT, 1988, 2 (12A) : 1570 - 1581
[6] INTEGRATION OF GENE MAPS - CHROMOSOME-1
COLLINS, A
KEATS, BJ
DRACOPOLI, N
SHIELDS, DC
MORTON, NE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) : 4598 - 4602
[7] TRANSCRIPTION OF THE HISTONE-H5 GENE IS NOT S-PHASE REGULATED
DALTON, S
COLEMAN, JR
WELLS, JRE
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (02) : 601 - 606
[8] DENT CL, 1991, J BIOL CHEM, V266, P20888
[9] DUNN IS, 1987, NUCLEIC ACIDS RES, V15, P2677
[10] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13

← 1 2 3 4 5 6 7 →