NON-CLATHRIN-COAT PROTEIN-ALPHA IS A CONSERVED SUBUNIT OF COATOMER AND IN SACCHAROMYCES-CEREVISIAE IS ESSENTIAL FOR GROWTH

被引：55

作者：

GERICH, B

ORCI, L

TSCHOCHNER, H

LOTTSPEICH, F

RAVAZZOLA, M

AMHERDT, M

WIELAND, F

HARTER, C

机构：

[1] MAX PLANCK INST BIOCHEM, GENZENTRUM, D-82152 MARTINSRIED, GERMANY

[2] UNIV GENEVA, SCH MED, DEPT MORPHOL, CH-1211 GENEVA 4, SWITZERLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 08期

关键词：

SECRETORY PATHWAY; COAT PROTEIN-COATED VESICLES; GOLGI MEMBRANES; WD-40; REPEAT;

D O I：

10.1073/pnas.92.8.3229

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To complete the molecular characterization of coatomer, the preformed cytosolic complex that is involved in the formation of biosynthetic transport vesicles, we have cloned and characterized the gene for non clathrin-coat protein alpha (alpha-COP) from Saccharomyces cerevisiae. The derived protein, molecular weight of 135,500, contains four WD-40 repeated motifs (Trp/Asp-containing motifs of approximate to 40 amino acids). Disruption of the yeast alpha-COP gene is lethal. Comparison of the DNA-derived primary structure with peptides from bovine alpha-COP shows a striking homology. alpha-COP is localized to coated transport vesicles and coated buds of Golgi membranes derived from CHO cells.

引用

页码：3229 / 3233

页数：5

共 40 条

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