HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION AND DIODE-ARRAY UV CONFIRMATION OF ISONICOTINIC-ACID HYDRAZIDE IN CATTLE MILK

被引：14

作者：

DEFILIPPI, A ^{[1
]}

PIANCONE, G ^{[1
]}

LAIA, RC ^{[1
]}

BALLA, S ^{[1
]}

TIBALDI, GP ^{[1
]}

机构：

[1] USSL 40,SANITA PUBBL LAB,I-10015 IVREA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00131-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the determination of isonicotinic acid hydrazide (isoniazid) in milk was developed. Milk was deproteinized with trichloroacetic acid. Isoniazid was condensed with cinnamaldehyde and assayed on a reversed-phase HPLC system, with good sensitivity and accuracy (10 mu g/l) with UV detection at 330 nm. Use of solid-phase extraction with a C-18 cartridge allows the detection limit to be lowered to 0.1 mu g/l with UV detection and confirmation of isoniazid hydrazone from the diode-array UV spectrum.

引用

页码：466 / 471

页数：6

共 13 条

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[2]

Avasthi R, 1989, J Assoc Physicians India, V37, P728

[3]

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[4]

EMELYANENCO KW, 1985, FARM ZH-KIEV, V2, P57