A phcnotypically normal male mouse, investigated because it was sterile, was found to possess 41 chromosomes. A karyotypic analysis indicated the extra element was one of the smallest chromosomes, almost certainly the Y. X-Y bivalcnts, Y-Y bivalents and X, Y, Y univalents were seen in meiotic metaphase I cells, and it was therefore concluded that the extra chromosomc was indeed the Y. Spermatogenesis tended to break down after meiosis I, but a few spermatids and sperm were observed. © 1969 S. Karger AG, Basel.