THE ACTION OF HYPOCHLOROUS ACID ON POLYMERIC COMPONENTS OF CARTILAGE

被引:52
作者
SCHILLER, J [1 ]
ARNHOLD, J [1 ]
GRUNDER, W [1 ]
ARNOLD, K [1 ]
机构
[1] UNIV LEIPZIG,INST MED PHYS & BIOPHYS,LIEBIGSTR 27,D-04103 LEIPZIG,GERMANY
来源
BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1994年 / 375卷 / 03期
关键词
CARTILAGE; CHONDROITINSULFATE; HYALURONIC ACID; HYPOCHLOROUS ACID; PROTON-NMR SPECTROSCOPY; RHEUMATOID ARTHRITIS;
D O I
10.1515/bchm3.1994.375.3.167
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The action of sodium hypochlorite on N-acetylglucosamine, N-acetylgalactosamine, chondroitinsulfate and hyaluronic acid was studied by H-1-nuclear magnetic resonance (H-1-NMR) in order to model some aspects of degradation processes caused by neutrophils on carbohydrate polymers of cartilage in rheumatoid arthritis. N-Acetyl side groups of carbohydrate monomers and chondroitinsulfate yield a resonance at 2.01-2.04 ppm in proton NMR-spectra. This resonance is observed in hyaluronic acid solutions only after a prolonged incubation to yield shorter polymeric chains. Sodium hypochlorite causes a continuous decrease of the line for N-acetyl groups. Two new resonances appear in the H-1-NMR spectra. An intermediate product, assumed as a chlorinated product of N-acetyl side chains, shows a chemical shift of about 2.35 ppm. This intermediate is hydrolyzed to a carbohydrate ring and acetate (1.90 ppm). Sodium hypochlorite acts in all systems investigated mainly on N-acetyl groups. Only small effects on the carbohydrate ring were found under our experimental conditions.
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页码:167 / 172
页数:6
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