Investigation of Stilbenoids as Potential Therapeutic Agents for Rotavirus Gastroenteritis

被引：16

作者：

Ball, Judith M. ^{[1
]}

Medina-Bolivar, Fabricio ^{[2
,3
]}

Defrates, Katelyn ^{[4
]}

Hambleton, Emily ^{[4
]}

Hurlburt, Megan E. ^{[4
]}

Fang, Lingling ^{[2
,3
]}

Yang, Tianhong ^{[2
,3
]}

Nopo-Olazabal, Luis ^{[2
,3
]}

Atwill, Richard L. ^{[2
,3
]}

Ghai, Pooja ^{[2
,3
]}

Parr, Rebecca D. ^{[1
,2
,3
,4
]}

机构：

[1] Texas A&M Univ, Dept Pathobiol, College Stn, TX 77843 USA

[2] Arkansas State Univ, Dept Biol Sci, Jonesboro, AR 72401 USA

[3] Arkansas State Univ, Arkansas Biosci Inst, Jonesboro, AR 72401 USA

[4] Stephen F Austin State Univ, Dept Biol, Nacogdoches, TX 75962 USA

来源：

ADVANCES IN VIROLOGY | 2015年 / 2015卷

基金：

美国国家科学基金会;

关键词：

D O I：

10.1155/2015/293524

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Rotavirus (RV) infections cause severe diarrhea in infants and young children worldwide. Vaccines are available but cost prohibitive for many countries and only reduce severe symptoms. Vaccinated infants continue to shed infectious particles, and studies show decreased efficacy of the RV vaccines in tropical and subtropical countries where they are needed most. Continuing surveillance for new RV strains, assessment of vaccine efficacy, and development of cost effective antiviral drugs remain an important aspect of RV studies. This study was to determine the efficacy of antioxidant and anti-inflammatory stilbenoids to inhibit RV replication. Peanut (A. hypogaea) hairy root cultures were induced to produce stilbenoids, which were purified by high performance countercurrent chromatography (HPCCC) and analyzed by HPLC. HT29.f8 cells were infected with RV in the presence stilbenoids. Cell viability counts showed no cytotoxic effects on HT29.f8 cells. Viral infectivity titers were calculated and comparatively assessed to determine the effects of stilbenoid treatments. Two stilbenoids, trans-arachidin-1 and trans-arachidin-3, show a significant decrease in RV infectivity titers. Western blot analyses performed on the infected cell lysates complemented the infectivity titrations and indicated a significant decrease in viral replication. These studies show the therapeutic potential of the stilbenoids against RV replication.

引用

页数：10

共 49 条

[1]

Purification of Resveratrol, Arachidin-1, and Arachidin-3 from Hairy Root Cultures of Peanut (Arachis hypogaea) and Determination of Their Antioxidant Activity and Cytotoxicity [J].

Abbott, Julie A. ;

Medina-Bolivar, Fabricio ;

Martin, Elizabeth M. ;

Engelberth, Abigail S. ;

Villagarcia, Hector ;

Clausen, Edgar C. ;

Carrier, Danielle J. .

BIOTECHNOLOGY PROGRESS, 2010, 26 (05) :1344-1351

[2]

Efficacy of pentavalent rotavirus vaccine against severe rotavirus gastroenteritis in infants in developing countries in sub-Saharan Africa: a randomised, double-blind, placebo-controlled trial [J].

Armah, George E. ;

Sow, Samba O. ;

Breiman, Robert F. ;

Dallas, Michael J. ;

Tapia, Milagritos D. ;

Feikin, Daniel R. ;

Binka, Fred N. ;

Steele, A. Duncan ;

Laserson, Kayla F. ;

Ansah, Nana A. ;

Levine, Myron M. ;

Lewis, Kristen ;

Coia, Michele L. ;

Attah-Poku, Margaret ;

Ojwando, Joel ;

Rivers, Stephen B. ;

Victor, John C. ;

Nyambane, Geoffrey ;

Hodgson, Abraham ;

Schoedel, Florian ;

Ciarlet, Max ;

Neuzil, Kathleen M. .

LANCET, 2010, 376 (9741) :606-614

[3]

Arnold M., 2009, CURRENT PROTOCOLS MI

[4]

Rotavirus NSP4: Aa multifunctional viral enterotoxin [J].

Ball, JM ;

Mitchell, DM ;

Gibbons, TF ;

Parr, RD .

VIRAL IMMUNOLOGY, 2005, 18 (01) :27-40

[5]

Resveratrol exhibits a strong cytotoxic activity in cultured cells and has an antiviral action against polyomavirus: potential clinical use [J].

Berardi, Valerio ;

Ricci, Francesca ;

Castelli, Mauro ;

Galati, Gaspare ;

Risuleo, Gianfranco .

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2009, 28

[6]

Efficacy of a monovalent human-bovine (116E) rotavirus vaccine in Indian infants: a randomised, double-blind, placebo-controlled trial [J].

Bhandari, Nita ;

Rongsen-Chandola, Temsunaro ;

Bavdekar, Ashish ;

John, Jacob ;

Antony, Kalpana ;

Taneja, Sunita ;

Goyal, Nidhi ;

Kawade, Anand ;

Kang, Gagandeep ;

Rathore, Sudeep Singh ;

Juvekar, Sanjay ;

Muliyil, Jayaprakash ;

Arya, Alok ;

Shaikh, Hanif ;

Abraham, Vinod ;

Vrati, Sudhanshu ;

Proschan, Michael ;

Kohberger, Robert ;

Thiry, Georges ;

Glass, Roger ;

Greenberg, Harry B. ;

Curlin, George ;

Mohan, Krishna ;

Harshavardhan, G. V. J. A. ;

Prasad, Sai ;

Rao, T. S. ;

Boslego, John ;

Bhan, Maharaj Kishan .

LANCET, 2014, 383 (9935) :2136-2143

[7]

Endocannabinoids in endocrine and related tumours [J].

Bifulco, Maurizio ;

Malfitano, Anna Maria ;

Pisanti, Simona ;

Laezza, Chiara .

[8]

Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site [J].

Bowman, GD ;

Nodelman, IM ;

Levy, O ;

Lin, SL ;

Tian, P ;

Zamb, TJ ;

Udem, SA ;

Venkataraghavan, B ;

Schutt, CE .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 304 (05) :861-871

[9]

Natural prenylated resveratrol analogs arachidin-1 and-3 demonstrate improved glucuronidation profiles and have affinity for cannabinoid receptors [J].

Brents, Lisa K. ;

Medina-Bolivar, Fabricio ;

Seely, Kathryn A. ;

Nair, Vipin ;

Bratton, Stacie M. ;

Nopo-Olazabal, Luis ;

Patel, Ronak Y. ;

Liu, Haining ;

Doerksen, Robert J. ;

Prather, Paul L. ;

Radominska-Pandya, Anna .

XENOBIOTICA, 2012, 42 (02) :139-156

[10]

Inhibition of herpes simplex virus infection by oligomeric stilbenoids through ROS generation [J].

Chen, Xiaoqing ;

Qiao, Haishi ;

Liu, Taixiang ;

Yang, Ziying ;

Xu, Lanfang ;

Xu, Yunxia ;

Ge, Hui Ming ;

Tan, Ren-Xiang ;

Li, Erguang .

ANTIVIRAL RESEARCH, 2012, 95 (01) :30-36

← 1 2 3 4 5 →