CRYSTALLIZATION OF A TYPE-II DEHYDROQUINASE FROM MYCOBACTERIUM-TUBERCULOSIS

被引：36

作者：

GOURLEY, DG

COGGINS, JR

ISAACS, NW

MOORE, JD

CHARLES, IG

HAWKINS, AR

机构：

[1] UNIV GLASGOW, DEPT BIOCHEM, GLASGOW G12 8QQ, SCOTLAND

[2] UNIV GLASGOW, DEPT CHEM, GLASGOW G12 8QQ, SCOTLAND

[3] UNIV NEWCASTLE UPON TYNE, DEPT BIOCHEM & GENET, NEWCASTLE UPON TYNE NE2 4HH, ENGLAND

[4] WELLCOME RES LABS, DEPT CELL BIOL, BECKENHAM BR3 3BS, KENT, ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 241卷 / 03期

关键词：

SHIKIMATE; QUINATE PATHWAYS; 3-DEHYDROQUINASE; CRYSTALLIZATION;

D O I：

10.1006/jmbi.1994.1524

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A type II 3-dehydroquinase from Mycobacterium tuberculosis has been crystallized in the presence of 6% polyethyleneglycol 6000. Data from these crystals have been collected to a resolution of 2.2 Angstrom on a rotating anode X-ray source. The space group has been determined as F23 with unit cell dimensions of a=b=c=127.8 Angstrom . There is one molecule in the asymmetric unit.

引用

收藏

页码：488 / 491

页数：4

相关论文

共 34 条

[1] THE SHIKIMATE PATHWAY - A METABOLIC TREE WITH MANY BRANCHES [J].

BENTLEY, R .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1990, 25 (05) :307-384

[2] CRYSTALLIZATION OF A TYPE-I 3-DEHYDROQUINASE FROM SALMONELLA-TYPHI [J].

BOYS, CWG ;

BURY, SM ;

SAWYER, L ;

MOORE, JD ;

CHARLES, IG ;

HAWKINS, AR ;

DEKA, R ;

KLEANTHOUS, C ;

COGGINS, JR .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 227 (01) :352-355

[3] SPECIFICITY OF ESCHERICHIA-COLI SHIKIMATE DEHYDROGENASE TOWARDS ANALOGS OF 3-DEHYDROSHIKIMIC ACID [J].

BUGG, TDH ;

ABELL, C ;

COGGINS, JR .

TETRAHEDRON LETTERS, 1988, 29 (51) :6779-6782

[4] THE ISOLATION AND NUCLEOTIDE-SEQUENCE OF THE COMPLEX AROM LOCUS OF ASPERGILLUS-NIDULANS [J].

CHARLES, IG ;

KEYTE, JW ;

BRAMMAR, WJ ;

SMITH, M ;

HAWKINS, AR .

NUCLEIC ACIDS RESEARCH, 1986, 14 (05) :2201-2213

[5]

CHAUDHURI S, 1991, BIOCHEM J, V275, P1

[6] PURIFICATION AND CHARACTERIZATION OF 3-DEHYDROQUINASE FROM ESCHERICHIA-COLI [J].

CHAUDHURI, S ;

LAMBERT, JM ;

MCCOLL, LA ;

COGGINS, JR .

BIOCHEMICAL JOURNAL, 1986, 239 (03) :699-704

[7]

DEKA RK, 1992, J BIOL CHEM, V267, P22237

[8] THE PENTAFUNCTIONAL AROM ENZYME OF SACCHAROMYCES-CEREVISIAE IS A MOSAIC OF MONOFUNCTIONAL DOMAINS [J].

DUNCAN, K ;

EDWARDS, RM ;

COGGINS, JR .

BIOCHEMICAL JOURNAL, 1987, 246 (02) :375-386

[9] PURIFICATION AND CHARACTERIZATION OF A DUAL FUNCTION 3-DEHYDROQUINATE DEHYDRATASE FROM AMYCOLATOPSIS-METHANOLICA [J].

EUVERINK, GJW ;

HESSELS, GI ;

VRIJBLOED, JW ;

COGGINS, JR ;

DIJKHUIZEN, L .

JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :2449-2457

[10] THE MYCOBACTERIUM-TUBERCULOSIS SHIKIMATE PATHWAY GENES - EVOLUTIONARY RELATIONSHIP BETWEEN BIOSYNTHETIC AND CATABOLIC 3-DEHYDROQUINASES [J].

GARBE, T ;

SERVOS, S ;

HAWKINS, A ;

DIMITRIADIS, G ;

YOUNG, D ;

DOUGAN, G ;

CHARLES, I .

MOLECULAR & GENERAL GENETICS, 1991, 228 (03) :385-392

← 1 2 3 4 →