MANIPULATION OF THE GENES FOR POLY-BETA-HYDROXYBUTYRIC ACID SYNTHESIS IN ALCALIGENES-EUTROPHUS

被引：15

作者：

PARK, HC ^{[1
]}

PARK, JS ^{[1
]}

LEE, YH ^{[1
]}

HUH, TL ^{[1
]}

机构：

[1] KYUNGPOOK NATL UNIV,COLL NAT SCI,DEPT GENET ENGN,TAEGU 702701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 07期

关键词：

D O I：

10.1007/BF00130359

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-beta-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.

引用

页码：729 / 734

页数：6

共 15 条

[1]

BIRHOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] MOLECULAR AND FUNCTIONAL-ANALYSIS OF THE TOL PLASMID PWWO FROM PSEUDOMONAS-PUTIDA AND CLONING OF GENES FOR THE ENTIRE REGULATED AROMATIC RING META-CLEAVAGE PATHWAY [J].